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proper inoculation technique Options
 
lorentz5
#1 Posted : 1/16/2011 8:51:07 PM

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In the PF tek, I am slightly confused:

I have seen this done differently in the growingmushrooms video, the official tek on the shroomery, and some posts here so I wanted to verify if this procedure would work:

1. poke 4 holes in metal lid, then close the jar with the lid and cover the holes with a piece of tape, then cover w/foil
2. PC, uncover foil, then inject syringe (with sterile technique) through the tape
3. take out of glovebox, remove tape

I see in those videos the jars are just sitting outside with the holes uncovered. Don't these holes expose to contaminants that we were so keen on avoiding?
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Myco
#2 Posted : 1/16/2011 9:04:25 PM

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If you are using the vermiculite barrier, recovering the holes is optional. Some folks cover the inoculation hole with micropore tape anyways. some folks also just use a band-aid.

If you weren't using the vermiculite barrier you would absolutely need to provide some kind of filter. in the lid.

The other questions I just answered in the stickied thread right after you posted this.
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lorentz5
#3 Posted : 1/18/2011 3:30:32 PM

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Thank you Myco, that more than adequately answers SWIM's questions (here and in the other thread). SWIM will likely be performing the KungPow tek here once he procures the funds for his first grow.
I am here
(1) For increased personal power, intellectual understanding, sharpened insight into self and culture, improvement of life situation, accelerated learning, professional growth.
(2) For duty, help of others, providing care, rehabilitation, rebirth for fellow men.
(3) For fun, sensuous enjoyment, aesthetic pleasure, interpersonal closeness, pure experience.
(4) For transcendence, liberation from ego and space-time limits; attainment of mystical union.
 
 
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