DMT-Nexus member
Posts: 198 Joined: 04-Mar-2009 Last visit: 27-Sep-2015 Location: That Great Gig in the Sky
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I wonder if theis process could also be applied to 5-ht? Jeeze, some one has to test all of these someday. I haven't heard reports on many, if any 4-ho, 4-po tryptamines other than psilocin and psyclobin (sp's?) By allowing this message to pass through your cornea, into your retina, you accept it as is and agree to my disclaimer regarding my posts that they are a complete falsification by doing so freeing me of all liability, direct, indirect, consequential or incidental that may arise from the instillation of this post in your memory bank.
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analytical chemist
   
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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some varieties (i.e. panaeolus) actually produce 5HT but there would be no point in adding it to psilocybe substrate; 4,5-dihydroxy-dmt probably isn't active, and psilocybe enzymes won't stop at bufotenine. "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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DMT-Nexus member
Posts: 442 Joined: 04-Sep-2009 Last visit: 12-Oct-2023
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DMT is good for expanding mushroom or LSD trips. I prefer smokin DMT 2-3 hours before droppings acid or eating mushrooms. works untill the next day i guess....  very intense CEVs i experienced
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DMT-Nexus member
Posts: 1926 Joined: 10-May-2009 Last visit: 27-Apr-2015 Location: ☂
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Does anyone have this paper available? Gartz demonstrates that P. cubensis produced high amounts of 4-HO-DET and minor amounts of 4-PO-DET from adding N,N-diethyl-tryptamine to the mycelial culture.
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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yeah, but what good is an experiment when no one is able repeat it? same with his other paper, adding tryptamine to substrate. interesting papers, but still inconclusive. "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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DMT-Nexus member
Posts: 1926 Joined: 10-May-2009 Last visit: 27-Apr-2015 Location: ☂
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Great, thank you! No one is able to repeat it, you say? Are we sure that these repetitions are done under the exact same conditions and the exact same way as Gartz? Do you by any chance have any paper available where there is attempted to repeat the experiment?
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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i've searched for such papers, but have yet to find one. and the folks over at the shroomery who speak of doing the experiments themselves never report any results. genetics is always a factor in these experiments, as are the growth parameters. some strains may have better expression of certain enzymes than others; thus, concentration of solutions would need to be adjusted accordingly. conc. is obviously very important as well "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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DMT-Nexus member
Posts: 161 Joined: 23-Oct-2009 Last visit: 30-Sep-2010
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Fuego wrote:whats the deal with this stuff... good information is hard to come by, and i havnt found any solid reports of people trying this. any information would be apreciated.
think for every hit of dmt you have having two trips of shrooms (50mg dmt --> 25mg psilocybin/psilocin X 2) i would say thats pretty fantastic, dont you agree? i have tried my hand at this before with much success... BUT the dmt must be in the substrate from the very beginning or your precious dmt will be wasted. i dissolved the dmt freebase in everclear and soaked it all up in a pf cake. casing methods with dmt in the substrate was less successful for me. i sterilized the cakes in a pot of boiling water careful to kill germs, but not kill dmt. 1.5g was ridiculous. edit---also check this out: http://www.shroomery.org...he-Potency-of-P-Cubensis"I'm creeping back to life, my nervous system all awry, I'm wearing the inside out. Look at him now, he's paler somehow, but he's coming round. He's starting to choke It's been so long since he spoke, well he can have the words right from my mouth. And with these words I can see, clear through the clouds that covered me, Just give it time then speak my name. now we can hear ourselves again" Pink Floyd- Wearing the Inside Out
Mogascreeta is a pathological liar and should not be taken seriously under any circumstance.
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The Great Namah
Posts: 3433 Joined: 18-Jan-2008 Last visit: 17-Sep-2020 Location: The place entites go when they smoke allspice
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Let me see if I understand what you are saying. You took DMT freebase and dissolved it in alcohol then let your PF cake absorb the DMT laden alcohol? I've spilt a bit of alcohol on both a cake and a casing before and neither of them ended up very well. The mycelium died wherever the alcohol hit it. Dunno, I'm glad it worked for you, but I think it would end up killing the cakes. Wouldn't it just be easier to convert to a salt, neutralize, and let the cake soak up the neutral pH water? The Spice extends life
The Spice expands consciousness
The Spice is vital for space travel
___________________________________________________________________________________________________
Never underestimate the power of STUFF!
I am certifiably insane, as such all posts written by me should be regarded as utter nonsense or attempts to get attention.
I don't know SWIM and personally don't trust him at all. If SWIM is posting, most likely I will not respond...as I said, I don't trust the guy. YOU I trust, but never SWIM.
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DMT-Nexus member
Posts: 937 Joined: 23-Oct-2009 Last visit: 25-Mar-2012 Location: Netherlands
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You are putting alcohol on mycelium?! Isnt alcohol used to kill microorganisms? Hmmmm “The most important thing in illness is never to lose heart.” -Nikolai Lenin
I know that you believe you understand what you think I said, but I'm not sure you realize that what you heard is not what I meant.
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DMT-Nexus member
Posts: 1926 Joined: 10-May-2009 Last visit: 27-Apr-2015 Location: ☂
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Maybe he ment that he soaked the substrate before adding the spores, ensuring that the alcohol was evaporated when the mycellium growth began?
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Nothing Stops The Void
Posts: 739 Joined: 19-Jun-2008 Last visit: 26-Nov-2013 Location: Blinded by the Lye
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Jeah ... he must have added the Ethanole before sterilizing , and even before moistening the substrate with water. Alcohol kills the mushroom -small Amounts cause Mutant Shrooms . Had he more quickly realized just who they were,he would have shown them more respect.Had he tried harder to fathom their brilliant minds,he would have taken more of their teachings to heart.Had he more clearly understood the purpose of their being,
he would have more vigorously tried to assist them.They were truly honorable; he was sadly prejudiced.
They were exceedingly well informed; he was grossly ignorant.They were totally indefatigable; he so often, and so quickly,gave up. Still, for many years there was a strong inter-species alliance between the Eleven-Eleven of the Half-way Realm, their Seraphic Associates,and their flesh-and-blood friend, a common mortal. Much was accomplished, many profited, and, there’s only one regret...They could have achieved so much more...
All Hypnotizing Hypnotizes Hypnotizing
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DMT-Nexus member
Posts: 227 Joined: 05-Jul-2009 Last visit: 17-Nov-2014
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What would you assume to be the best way to go about conducting an experiment on this? SWIM was thinking of inoculating a few jar with some DMT fumarate, do you think this would work? Should he instead try the alcohol soak method? He might try both. He was gunna use some pure white spice, but he also considered trying one with some jungle. How many mgs do you think would be needed to add to the substrate and what would be the best method of adding the spice? Step forward into your cave. That's right. You're going deeper into your cave. And you're going to find, your power animal...
Imagine your pain as a white ball of healing light. It moves over your body, healing you. Now keep this going, remember to breathe, and step forward through the backdoor of the room. Where does it lead?
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DMT-Nexus member
Posts: 210 Joined: 25-Nov-2009 Last visit: 10-Jun-2013
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mattritt wrote:What would you assume to be the best way to go about conducting an experiment on this? SWIM was thinking of inoculating a few jar with some DMT fumarate, do you think this would work? Should he instead try the alcohol soak method? He might try both. He was gunna use some pure white spice, but he also considered trying one with some jungle. How many mgs do you think would be needed to add to the substrate and what would be the best method of adding the spice? Well DMT fumarate is soluable in water, so you could just add some to the water you use when making up the substrate. How much I don't really have a good feel for. Maybe try several different amounts ranging from 0.01 to 1% by weight of the water? elphologist
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DMT-Nexus member
Posts: 57 Joined: 26-Jul-2009 Last visit: 17-Mar-2015 Location: Shpongland
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Sorry to bring up old topic, but just found this: Quote:However there is a very interesting study that took place in Leipzig about 15 years ago. Jochen Gartz, a mushroom explorer whom I know quite well, has done some fascinating studies with Psilocybe species by raising them on solid media containing strange tryptamines that are alien to the mushroom. Apparently the enzymes that are responsible for the 4-hydroxy group of psilocin are indifferent to what it is they choose to 4-hydroxylate. He has taken things like DPT or DIPT and put them in the growth media and the fruiting bodies that came out contain 4-hydroxy-DPT or 4-hydroxy-DIPT instead of psilocin. In fact, he has a patent on the process. These active compounds are made by the mushroom so they really are natural and yet they never have been observed in nature. I'll give you even odds that if you put spores of a psilocybe species on cow droppings loaded with 5-MeO-DMT you would come out with mushrooms containing 4,5-HO-MeO-DMT. This way you avoid a 10 step synthesis by growing a psychoactive mushroom that contains no illegal drug.
That is Alexander Shulgin talking....sooooo................... Also, link to here I found that here. “I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
-Watts-
"We live together, we act on, and react to, one another; but always and in all circumstances we are by ourselves. The martyrs go hand in hand into the arena; they are crucified alone. Embraced, the lovers desperately try to fuse their insulated ecstasies into a single self-transcendence; in vain. By its very nature every embodied spirit is doomed to suffer and enjoy in solitude. Sensations, feelings, insights, fancies — all these are private and, except through symbols and at second hand, incommunicable. We can pool information about experiences, but never the experiences themselves. From family to nation, every human group is a society of island universes."
-Huxley-
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DMT-Nexus member
Posts: 322 Joined: 05-Jul-2009 Last visit: 14-Jul-2024
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Another approach would be a simple Limetek; and then after pulling with D-limo, salt with vinegar, and use that solution to hydrate the perlite component of a PF tech.:idea:
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DMT-Nexus member
Posts: 1052 Joined: 18-Jan-2008 Last visit: 29-Jun-2017 Location: Earth, of course??
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plumsmooth wrote:Another approach would be a simple Limetek; and then after pulling with D-limo, salt with vinegar, and use that solution to hydrate the perlite component of a PF tech.:idea: ....*vermiculite component And this might make the substrate more acidic...? We usually want to make it slightly basic if anything, so that nasty growths are discouraged and our intended cultures can still grow. "Within your heart is a lotus, and within this lotus is a diamond. This diamond is the source of creation, and in all the creation, there is only one lotus."
"Only from the Heart can you touch the sky." ~ Rumi
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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exactly this is the reason people add lime to their casing, to make it slightly basic to ward off contaminants. the best addition to a substrate would probably be NMT rather than DMT. the old Gartz paper showed greater turnover for NMT than the next best one, tryptamine. "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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