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contaminated spore syringe? Options
 
psychosisdoses
#1 Posted : 5/29/2009 11:06:39 PM

Derek


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greetings all..
quick (maybe) question..
i received my syringe today and was excited to make some slides and study them under 400x... when i noticed there was a black clump in the syringe kinda just floatin around... i wasnt sure if this was normal

any idears?
psychosisdoses attached the following image(s):
spor.jpg (30kb) downloaded 225 time(s).
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 

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Buster
#2 Posted : 5/29/2009 11:17:44 PM

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I have only recieved a small amount of spore syringes in my time, but they have all been almost just like water inside,
untill closer inspection. none have been like whats in the pic you show.

peace.
I'm the one that's got to die when it's time for me to die, so let me live my life the way I want to.




 
amor_fati
#3 Posted : 5/29/2009 11:36:14 PM

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SWIM says that's just a clump of spores. Shake it up before use.
 
Buster
#4 Posted : 5/30/2009 12:02:52 AM

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Every day is an education in here.
Thats well noted for futureVery happy
I'm the one that's got to die when it's time for me to die, so let me live my life the way I want to.




 
psychosisdoses
#5 Posted : 5/30/2009 5:57:28 AM

Derek


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i feel silly now Embarrased
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
#6 Posted : 5/30/2009 12:06:21 PM
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Yup, just a clump o' spore. Some of SWIMs will do that when they sit for awhile.. But yeah just shake it up real good and inject!!


*COMPASSION N' RESPECT'
 
psychosisdoses
#7 Posted : 6/1/2009 6:59:54 AM

Derek


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THAT DAMN SYRINGE IS POSSESSED!!!
it levitated and penetrated 36 holes of the nine 1/2 pint jars of substrate.. ill keep the jars as a memento of this bizarre incident... Laughing
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
acolon_5
#8 Posted : 6/1/2009 3:06:30 PM

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Lol, keep us updated on that strange and mysterious incident. Who knows what could happen when the paranormal and spore syringes mix!

(BTW, that is totally a huge clump of spores, damn that's a lot of spores in one syringe)
The Spice extends life
The Spice expands consciousness
The Spice is vital for space travel
___________________________________________________________________________________________________
Never underestimate the power of STUFF!


I am certifiably insane, as such all posts written by me should be regarded as utter nonsense or attempts to get attention.

I don't know SWIM and personally don't trust him at all. If SWIM is posting, most likely I will not respond...as I said, I don't trust the guy. YOU I trust, but never SWIM.
 
psychosisdoses
#9 Posted : 6/9/2009 9:58:48 PM

Derek


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9 days so far temps between 72-80 degrees in cardboard box
they all look about like this
some more some less
so far they are all white clumps no black or green or anything
ahhhh seems to be lookin good Very happy
psychosisdoses attached the following image(s):
mycel.jpg (43kb) downloaded 176 time(s).
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
benzyme
#10 Posted : 6/9/2009 10:10:46 PM

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9 days?

looks like it may need some fresh air exchange, but looking good so far. are there breather holes?
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
psychosisdoses
#11 Posted : 6/9/2009 10:16:35 PM

Derek


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benzyme wrote:
9 days?

looks like it may need some fresh air exchange, but looking good so far. are there breather holes?


ohhh i didnt know i had to... i covered the inoc holes with transpore tape replaced the foil and screwed the band back on
should i take off the foil .. inadequte air exchange that would just slow it down right not hurt it?
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
benzyme
#12 Posted : 6/9/2009 10:36:42 PM

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that's right

if a layer of dry vermiculite was added to the top of each cake, it acts as a barrier against contaminants; the holes could even be left uncovered, or covered with medical breather tape
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
psychosisdoses
#13 Posted : 6/9/2009 10:48:39 PM

Derek


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benzyme wrote:
that's right

if a layer of dry vermiculite was added to the top of each cake, it acts as a barrier against contaminants; the holes could even be left uncovered, or covered with medical breather tape


yes these jars have the layer of vermiculite i will go ahead and remove the foil thanks its good thing i posted this and you were there for me Smile

should i open the box they are in once a day as well will the brief exposure to light hurt
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
psychosisdoses
#14 Posted : 6/9/2009 10:55:30 PM

Derek


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this was the most advanced of them
foil was removed from lids
one or two of the jars only had a few small spots
psychosisdoses attached the following image(s):
mycellyyy.jpg (41kb) downloaded 157 time(s).
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
benzyme
#15 Posted : 6/10/2009 12:55:45 AM

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psychosisdoses wrote:

should i open the box they are in once a day as well will the brief exposure to light hurt


the light won't hurt at all.
though once they're fully colonized, the light will actually trigger pinning


"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
psychosisdoses
#16 Posted : 6/10/2009 2:03:02 AM

Derek


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benzyme wrote:
psychosisdoses wrote:

should i open the box they are in once a day as well will the brief exposure to light hurt


the light won't hurt at all.
though once they're fully colonized, the light will actually trigger pinning




this is what i understood
i would want to expose them to light 4-12 hours a day at that point
how long should i let them pin like that before birthing?
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
benzyme
#17 Posted : 6/10/2009 2:06:26 AM

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psychosisdoses wrote:
benzyme wrote:
psychosisdoses wrote:

should i open the box they are in once a day as well will the brief exposure to light hurt


the light won't hurt at all.
though once they're fully colonized, the light will actually trigger pinning




this is what i understood
i would want to expose them to light 4-12 hours a day at that point
how long should i let them pin like that before birthing?


when they form small primordia (as soon as the heads turn reddish, carefully birth them)
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
psychosisdoses
#18 Posted : 6/10/2009 5:32:33 PM

Derek


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sweet will do...
SOOOOO EXCITED!! Smile


for my birthin chamber i got this

24.2 x 16 x 12 inches

i also have 8qt of perlite
i want an inch layer on the bottom correct?
how much water should i soak it with should i just put the perlite in a strainer to soak it or lay it and pour water in?
before i use the box i suppose i should spray it all down with lysol..
for air exchange should i just remove the lid and fan it once a day or make small holes and cover with transpore tape?
one more question Razz when i birth em should i put em on a peice of foil (that was wiped down with alcohol) ontop of the perlite.. and does it matter if its bottoms up or down which is preferred?

thanks for all your help
"once youve locked yourself into a serious drug collection the tendency is to push it as far as you can..." - hunter s. thompson

~~~~~~~~...You are me and i am you, i will always be with you...~~~~~~~~IAmUsWeYouMe~~~~~~~~
‹maxzar100› YOU are like acid
‹mattimus› dosesdosingdoses
 
Pebble on the Beach
#19 Posted : 6/10/2009 5:47:07 PM

C r a c k B l i p T o o t T o o t ! ! !


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psychosisdoses wrote:
sweet will do...
SOOOOO EXCITED!! Smile


for my birthin chamber i got this

24.2 x 16 x 12 inches

i also have 8qt of perlite
i want an inch layer on the bottom correct?
how much water should i soak it with should i just put the perlite in a strainer to soak it or lay it and pour water in?
before i use the box i suppose i should spray it all down with lysol..
for air exchange should i just remove the lid and fan it once a day or make small holes and cover with transpore tape?
one more question Razz when i birth em should i put em on a peice of foil (that was wiped down with alcohol) ontop of the perlite.. and does it matter if its bottoms up or down which is preferred?

thanks for all your help



just got the same from some swedish design chain (you all know it). they got everey size and shape your heart could desire.
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and
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Bertrand Russell

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benzyme
#20 Posted : 6/10/2009 5:49:04 PM

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it all sounds good, but go with at least a couple inches of perlite; otherwise the water may evaporate too quickly.
swim uses about 3-4 inches.

doesn't matter what side of the foil faces up, its purpose is to prevent the cake from getting too wet resulting in rotting.

everything else sounds about right. give the chamber a couple good sprays of lysol, after adding perlite (which you can just wet through a strainer..no need for a layer of water at the bottom), and wait a few minutes to disperse. you can then transfer the cakes to the pieces of foil on top of the perlite, the lysol won't really affect them.

the trick is to make sure the cakes are as fully colonized, as much as possible, before transferring to the fruiting chamber. this is the primary defense against contamination, since airborne contaminants will compete for the substrate.

after transferring, spray the whole chamber with water two or three times a day, and fan about four or five times a day
"Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
 
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