I was wondering if there is some evidence of mushrooms containing dmt? I know psilocybin is very closely related to dmt, but is there actual dmt in a mushroom organism?

AFAIK there is no evidence that nn has been found in any mushroom species. I'm willing to bet we will however, eventually find one.

DMT is in its chemical name
4 PO DMT (O-phosphoryl-4-hydroxy-N,N-dimethyltryptamine)
but as anrchy said no N,N DMT by itself discovered yet.

Just a speculative question, would it be possible to make DMT from psilocybin?

Personally, I'd just take the shrooms for the psilocybin and source the DMT from the non-fungi world, but just out of curiousity

This borders on synth talk, which is banned discussion here on The Nexus, just fyi.

Psilocin (4-HO-DMT) is a bit of a finicky molecule to work with, chemically speaking, and I'm not sure there's anything you could do to the hydroxyl group to remove it that wouldn't also damage other parts of the molecule. Even getting psilocin extracted and isolated has proved to be really difficult (there are some discussions on it over in the Enhanced Chemistry forum).

Assuming you even could get pure psilocin, I have wondered about the possibility to using some kind of acid to protonate the hydroxyl and then extracting the (hypothetical) remaining N,N-DMT into a NP solvent like naptha, but I feel like if it were that simple someone else would have come up with it before me.

Yeah, does sound like a struggle, and ultimately a little pointless.

Like I said, I was just wondering aloud if it was actually possible

I wouldn't say pointless. If it could be done, it would certainly be useful, both from a logistical standpoint (no need to repeatedly buy shipments of legally grey plant matter over the web), and also environmentally (no need to harvest potentially endangered plants).

I'm just spitballing here, but I think the biggest breakthrough will be when we can take the gene sequences that encode the metabolic pathway for DMT synthesis out of, say, Mimosa, and insert them into some kind of generic, easy-to-grow mushroom (like wood-ear mushrooms, for example).

That will change everything.

I was thinking about this the other day, that maybe those genes could be inserted into related plants/trees that could be grown in more varied climates than mimosa or acacia (e.g. albizia). Though, I guess mushrooms or perhaps grass would be more sustainable and provide bigger harvests for shorter growing seasons. I wonder if it would be possible to just use bacteria to do the work instead? Then you would just give the bacteria large amounts of a (sterile) substrate to grow on, and incubate.

Bacteria or algae could work as well. The problem isn't finding a suitable host, but rather, figuring out 1) which genes encode the enzymes that make the biosynthesis of DMT possible, 2) how to remove them from the original life form and 3) how to effectively insert them into our host of choice.

That's the tricky part.

In theory, this could do a lot of damage to the pharmaceutical industry: any medicines that re extracted from natural sources (eg morphine) could be made by the literal bucketful in a basement, with minimum expenditure of resources. No big grow operations, just a fish tank and some substrate.

If the techniques were available to the average person, it could also cripple the War On Drugs.

Even if it were to turn out that a certain mushroom did produce a certain percentage of N,N DMT, it's not like it would have an effect on the user since DMT isn't orally active anyway.

I think the idea is that mushrooms are easier to grow, so it would make bulk extractions more feasible/safer.

fungi fix phosphorus in the soil, so that plants can then uptake it. Sort of similar to how legumes are nitrogen fixers. Without the fungi there would not be phosphorus for the plants..this is why the tryptamines in fungus are phosphorylated..and why they end up with high levels of psilocybin and not DMT...

I was under the impression however, that there is minor ammounts of DMT in psilocybe mushrooms and other species. Not usable ammounts..am I wrong?