pH 11 is WAY TOO HIGH. If using calcium hydroxide, the bufotenine will start to decompose. Shoot for 8.5-9.5 and don’t go any higher.

Calcium hydroxide and also ammonium hydroxide (and very likely sodium hydroxide) can decompose bufotenine at pH 11, so be careful not to let the pH get too high. Sodium carbonate can’t decompose bufotenine at pH 11 according to SWIM’s tests.

Edit: Okay, SWIM added limonene to his dark brownish powder and simmered for about 3 minutes.. He let it stop simmering for a few seconds and poured the limonene through a coffee filter into a pan container. The only thing that really got in the filter was a little bit of the brown powder. So the bufotenine is supposed to "fall" out of this cooling limonene? It's been about 50 minutes and SWIM sees nothing but the limonene. He just has this and the brown powder in his stovetop pan..

So after an hour of cooling he is to take just the limonene in the container and simmer that again in the powder, and repeat filtering back into the container? Then the powder is trash?

SWIM thinks something didn't work. He followed all the steps, but used 4g seeds, 200ml water, 25ml 5% acidity vinegar, 800mg sodium carbonate, and 30ml limonene. What's the final product supposed to look like?

what do you all think about a dry tek a/b on the seeds using boiling xylene both times?

mapp..the filtering is probably filtering out all the bufo..so maybe if you soak it in acetone or alcohol you may be able to get it out...

boiling xylene is fucking GROSS! d-limonene is the only thing SWIM will boil now

well, in concept will it pull it out nice and pure or leave a bunch of gook?

mapp, that sucks...

I think Jorkest is right in that a good chunk of the bufotenine's probably in the filter. SWIY's expected yield would probably be ~100 mg, which is a _really_ small amount of stuff in absolute terms, easily small enough, I would think, that it could all be trapped in the filter.

SWIM tells me he didn't filter the limonene, he just decanted as much as possible (Which was most of it, the junk stayed pretty well at the bottom). That's why he did it twice, so that the amount of bufo left in the d-limonene he was unable to pour out from simmering container along with the nasty junk would be minimal.

yes it would clean it very nicely..BUT...you will have trace amounts of xylene in it..

69ron's FOAF ate 100mg of xylene boiled bufotenine to test it out..and he said he was burping up xylene...which is gross..

so if you were to do a xylene boil...you would then want to take your crystals and put them in some acetone..and THEN let that evap..because whats going to happen is that the acetone will break apart the crystal lattice..and free the xylene caught in there...then you would evaporate the acetone..and you will be left with probably not as pretty crystals..but they should have a significantly less xylene content

Dagger, could you please report back on how SWIY's end product was?

charcoaled?

Hmm, that's frustrating.

I guess sticking to Jorkest's method is the way to go.

ahh its just one test...SWIM is still trying to figure out a simple way of purifying the bufo from d-limo tek further..because he vaporized some in a test tube and it left some residue..not sure what it is..but hes gonna see if he can grow some crystals in some slowly evaporating MEK...and will try acetone and maybe some ethanol..

that should purify it a LITTLE bit..

the reason he says this..is because when SWIM puts some of his bufo in MEK..there seems to always be little something that doesnt get absorb by it...which is strange...because this stuff came out of boiling d-limonene...

so what he did was he took all his bufo..yes all of it...and put it in a shot glass full of MEK...it may have been around 2g bufotenine in about 30ml MEK...the MEK turned very amber in color...he let everything settle...and then poured the amber MEK into another shot glass..then..with the solids that didnt get absorbed he poured another 20ml of MEK on top of them...now the interesting part is that there are still solids on the bottom..BUT the MEK is almost more yellow than amber now..its extremely light compared to the first one..so something is happening here that SWIM cant explain..

either the first MEK pull got more of the toxins out..and left the cleaner bufo...or it took the bufo and left more toxins that arent soluble in MEK...its hard to say...so SWIM is going to let these two different pulls evap..and see which one comes out cleaner..and then he'll have to test them..

he will probably do one more pull on the solids and see if he can get anything else to come out...

SWIM would have to say..that bufotenine purification is one difficult thing to do...SWIM can do the same EXACT thing three different times and come up with three different purities...

he can do a MEK:heptane pull once..and end up with fluffy white crystals...and he hasnt been able to do it again...not once...



EDIT: so the third pull..SWIM says is even LIGHTER than the second almost clear..but there are still solids..what are these solids..and where did they come from..and its not like there are just a few little chunks..it could be something like half a gram of material..

so SWIM is going to evaporate that MEK and see what comes out..and then dry out those solids to see what color they are..and what they smell like when vaporized...because SWIM personally is through with smoking toxins

ahh...and you know somebody who then just ate the seeds and had a nice experience!? that is interesting indeed...

SWIM did a CASA (citric acid) on previously limonene extracted dark stuff. A lot of stuffed precipitated (around 1g), SWIM was excited... the precipitate appeared to be a dark sticky gum. SWIM added some calcium carbonate, wet, dried and put it in boiling limonene.
Limonene was evaporated and left less than 300 mg of something that did'nt want to fully dry. The limonene was not able to dissolve a glossy tar that remains.
Bioassay showed that the brown gum stuff is somehow psychoactive (seems less active that a previous naphta:MEK extract), but burning it showed also that a noticeable amount of material ashes remained so the limo->CASA->limo is far to be a pure product and it is quite harsh on SWIM lungs.
Getting fluffy crystals is still a dream for SWIM...

ive got a pretty good method for breaking the crystal structure and releasing xylene trapped in dmt and jungle spice extractions... you just get an id card or something and scrape it when its still slightly wet and then wipe it back on the pan wait for a little and do it again and again until it gets harder to scrape... it should turn a lighter color too... keep doing this until its flakey and has a soap consistancy and all the xylene should be out. hope it works for bufo... someone should be getting some seeds in today

also, would bufo acetate be soluble in acetone?

Dagger,

That's probably true. SWIM just doesn't like other people potentially getting the error part of the trial-and-error inherent to refining a tek, especially if a tried-and-true method is out there. SWIM somewhat wishes in hindsight that he had perfected the tek a bit more before posting.

Also, the word "tarry" for SWIM conjures up a black tarry extract that produced nasty projectile vomiting when bioassayed. Not good memories.

hahaha Spaz...thats funny...so much puking when testing out bufo extracts for the first times..ahhahahaha makes SWIM laugh...not at you...but at his own memories of it...hehehehehe

and not sure about bufo acetate...might be a good test..

and xylene almost tends to stick to the alkies...but SWIM has used that method before to dry crystals..but there will always be a hint of solvent in anything you extract...so its best to finish with a solvent you arent to worried about ingesting minute amounts of

SWIM just did a tek posted by spasticspaz in the Smoking Colubrina Seeds thread in Other Entheogens, to his own honest provisions:

Cracked/dried 10g seeds in oven at 250F for 45 min, then at 450F for 15 min
Added ~3g(?) calcium hydroxide (eye balled 1/3 versus 10g crushed seeds)
Added (too much) water to the mix (it was liquidy)
But Evaporated/Dried in oven at 130F for 40 minutes, then at 450F for 10 min
Moved to a glass container with a lid
Added 190ml acetone to the mix and shaked periodically for 1 hr
Decanted acetone into a dish
Added 180ml acetone to the mix and shaked periodically for 1 hr
Decanted acetone with previous acetone into dish (this time when all the liquid was pouring, some of the powder that was not sticking to the bottom of the glass was basically impossible not to follow in the acetone..a coffee filter was not proving to help at all.. this wouldn't make a big difference in the tek after the forthcoming evaporation would it? ..SWIM experienced this same "predicament" with previous bufo tek attempts...)
Evaporated in oven at 200F for 2 hours


After evaporating at the end for 2 hours what is left is tar-ish, and glossy in the middle, not unlike how described previously in this thread... But SWIM put the dish back in the oven at only 100-250F for about 4 hours to try to get it drier, and then scraped - it's really a kind of thick syrupy viscosity that won't run, but doesn't seem collectable. SWIM can't really pick it up or anything, it just sticks to whatever touches it. Will it eventually dry out in the oven at this temp?

So is this actually a crude bufotenine extract? Smokable, snortable, pluggable , but not effective orally, right

Also, after have used the aforementioned tek, can SWIM use food grade d-limonene on his yield to any purifiying effect, or would it be a waste/damaging? ..Would he just simmer a small amount, add the bufotenine for 5 minutes, turn off heat, then toss the limonene & keep the tar?

What you have there will be bufotenine with other alkaloids and toxins.

I'd highly recommend purifying further.

SWIM tried smoking an impure extract and it wasn't making him nauseous but it wasn't potent enough to do the job.

Ah. SWIM wasn't sure of the present toxin amount, as he had read that toasting the seeds before and after basifying removes the toxins. Although... SWIM only "toasted" at 450F in the oven for about 15 minutes each time..

But, as SWIY implies, purifying SWIM's extract would still make this more potent. How exactly, if possible, would SWIM purify this with limonene? He wouldn't want to screw up this late, and may just attempt purifying part of his yield..