I just watched the video of RoadKill about grain to grain transfer and I learned that you need to be very sterile when you transfer the colonized rye grain to the non-colonized grain. But then I started to watch Lets do straw and he just open the jar and empty it in the straw without anything sterile. (makes sens since the mycelium should be strong enough to defend itself. So then why is it important to be sterile when transfering rye grain to another jar?!?!!?!?!

I think its somewhat related/dependant on how much colonized material to uncolonized material you will be using, but onviously the cleaner the better

please use the search option on the shroomery first.
Rye to rye is usually done for making more spawn. Spawn needs to be sterile to prevent contams later on.
Rye to straw is done only to colonize the straw for producing fruits. Usually the straw is pasteurized, so it's not sterile at all, it just gives your spawn an advantage.
The reason to use spawn is to colonize the medium extra fast by providing allot of inoculation points(read grain kernels)in order to make sure that only the wanted fungus will win the race of colonization.

hope this helps

Don't confuse a grain to grain transfer with innoculating pasteurized substrate.

In G2G you're putting colonized grain into a jar (should be sterile - at least that's what you're shooting for) of other grain that will stay as sterile when you put the cap back on after the transfer.

When innoculating substrate, you're mixing that grain spawn with a pasteurized substrate (straw, coir, poo, etc) and then leaving it in an environment that will not be sterile (monotub, etc.).


I do G2G transfer in a still air box and rarely have any issues. Doing it in front of a laminar flow hood would be optimal.

Reading up on sterile procedure will clear up a lot of these type of questions

Thank you very much for all that information!