ThGiL fO TiRipS
Posts: 2021 Joined: 26-Feb-2011 Last visit: 07-Feb-2023 Location: Earth
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After about two months waiting my B+ spores arrived I am very happy but I will need your help now My plan is to go for PF tek easy for beginners. I have all the tools I need. I guess I know what I am doing the only thing I wanted to ask about how much of spore suspension do you guys normally use per one jar. I read it should be 1 to 4 ml. What is your experience ? Also how do I know how much of spore suspension should I make out of my prints ? How do I know when it is enough prints in sterile water ? Should I use all my prints for making my spore syringe ? Thank you friends We are each of us angels with only one wing, and we can only fly by embracing one another.
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We are all living in our own feces.
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DMT-Nexus member
Posts: 653 Joined: 19-Dec-2008 Last visit: 31-Dec-2018
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You only need about 1cc per jar. Put a 1/4 cc in all 4 corners of the jar.
As far as making spore syringes out of prints, you only need to scrape about a max of 25% of the print. That will contains a ton of spores...you can't see them. Less is more.
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DMT-Nexus member
Posts: 314 Joined: 31-Jan-2012 Last visit: 04-Feb-2017
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I am 6 weeks into this exact process: B+ spores and the PF Tek. I received ready-to-inoculate syringes, however, requiring no extra preparation. I officially inoculated my jars on the 3rd of March or several days earlier and right now I have six cakes in a shotgun terrarium. No pinning as of yet, but I am confident it is only a matter of days. I will be making prints and syringes as well. For what it's worth, I have had no contamination, although things are running slightly behind schedule. I'll contribute whatever knowledge I can to your cause. "Culture is NOT your friend" - TMK
Dead-Yolk-Mau5 - Yolks N' Stuff ( 2008 )
The year is 01 ADMT
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DMT-Nexus member
Posts: 653 Joined: 19-Dec-2008 Last visit: 31-Dec-2018
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I definitely prefer to do bulk grows....but I think the pf tek is a nice place for new people to start.
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ThGiL fO TiRipS
Posts: 2021 Joined: 26-Feb-2011 Last visit: 07-Feb-2023 Location: Earth
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behindthelight wrote:You only need about 1cc per jar. Put a 1/4 cc in all 4 corners of the jar.
As far as making spore syringes out of prints, you only need to scrape about a max of 25% of the print. That will contains a ton of spores...you can't see them. Less is more. Thank you that is the info I needed We are each of us angels with only one wing, and we can only fly by embracing one another.
*********
We are all living in our own feces.
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ThGiL fO TiRipS
Posts: 2021 Joined: 26-Feb-2011 Last visit: 07-Feb-2023 Location: Earth
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un-known-ome wrote:I am 6 weeks into this exact process: B+ spores and the PF Tek. I received ready-to-inoculate syringes, however, requiring no extra preparation. I officially inoculated my jars on the 3rd of March or several days earlier and right now I have six cakes in a shotgun terrarium. No pinning as of yet, but I am confident it is only a matter of days. I will be making prints and syringes as well. For what it's worth, I have had no contamination, although things are running slightly behind schedule. I'll contribute whatever knowledge I can to your cause. Congrat for that, do not forget to take some pics for us By the way did you use pressure cooker or did you steam sterilize in a pot ? We are each of us angels with only one wing, and we can only fly by embracing one another.
*********
We are all living in our own feces.
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13.7 Billion Year Old Noob
Posts: 182 Joined: 16-Aug-2011 Last visit: 19-Mar-2022 Location: Africa
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all the best!
growing mushrooms is incredibly rewarding...
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DMT-Nexus member
Posts: 1760 Joined: 15-Apr-2008 Last visit: 06-Mar-2024 Location: in the Forest
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Good luck , be patient, work carefully. Be over the top on sterility procedures, mold sucks The only way of discovering the limits of the possible is to venture a little way past them into the impossible. Arthur C. Clarke http://vimeo.com/32001208
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DMT-Nexus member
Posts: 314 Joined: 31-Jan-2012 Last visit: 04-Feb-2017
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smokerx wrote:un-known-ome wrote:I am 6 weeks into this exact process: B+ spores and the PF Tek. I received ready-to-inoculate syringes, however, requiring no extra preparation. I officially inoculated my jars on the 3rd of March or several days earlier and right now I have six cakes in a shotgun terrarium. No pinning as of yet, but I am confident it is only a matter of days. I will be making prints and syringes as well. For what it's worth, I have had no contamination, although things are running slightly behind schedule. I'll contribute whatever knowledge I can to your cause. Congrat for that, do not forget to take some pics for us By the way did you use pressure cooker or did you steam sterilize in a pot ? Thank you. I wasn't sure what I was getting myself into but it's already been a thoroughly rewarding project and I haven't even flushed my cakes yet. I opted for fractional sterilization in a steel pot over the course of three days. I believe that particular method might be superior to the pressure cooker, but either way will work. It's not an exact science or anything, so just set a burner on 3 or below and sterilize for over an hour approximately ever 24 hours. Once you've done that, leave the jars out for at least another day or perhaps longer to observe possible contamination. If steam sterilizing, be certain that there is a high enough water level that it does not all boil off, and also that you have lids placed beneath the jars. "Culture is NOT your friend" - TMK
Dead-Yolk-Mau5 - Yolks N' Stuff ( 2008 )
The year is 01 ADMT
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ThGiL fO TiRipS
Posts: 2021 Joined: 26-Feb-2011 Last visit: 07-Feb-2023 Location: Earth
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Thank you guys I will be very careful not to f..k it up Cant wait to start but now I have to wait for more then a months cos I am going away in few weeks and would not be able to be here for whole time. Anyway I waited so long I can wait a bit longer those spores will not run away Patience is the key We are each of us angels with only one wing, and we can only fly by embracing one another.
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We are all living in our own feces.
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DMT-Nexus member
Posts: 653 Joined: 19-Dec-2008 Last visit: 31-Dec-2018
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un-known-ome wrote:I opted for fractional sterilization in a steel pot over the course of three days. I believe that particular method might be superior to the pressure cooker, but either way will work.
nothing is better than a pressure cooker.....by far the best way to sterilize
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DMT-Nexus member
Posts: 143 Joined: 14-Jan-2012 Last visit: 04-Dec-2015 Location: everywhere and nowhere
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behindthelight wrote:un-known-ome wrote:I opted for fractional sterilization in a steel pot over the course of three days. I believe that particular method might be superior to the pressure cooker, but either way will work.
nothing is better than a pressure cooker.....by far the best way to sterilize I just bought a pressure cooker from big lots for 20$. Worth it IMO. I still havn't decided what strain I want though thinking either B+ or H3. Anyway good luck to all. Love + Light "for as long as there is love and light; I will fight for what is right; as a warrior with all my might; I will guarantee that hope shines bright" --Prayer of the Paladin "If you labor, you are a "laborer", If you work on a farm, you are a "farmer", If you flow, you are a "flower""--Forest Sage Community, Love and Passion
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DMT-Nexus member
Posts: 653 Joined: 19-Dec-2008 Last visit: 31-Dec-2018
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Yeah man, I have a $20 pressure cooker too and the thing works perfect. I have prepped a lot of grain jars and grain bags in it and it has worked every time with no issues.
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