been awhile since visited, thanks

Would a SEP FUNNEL be a easier way?

Which is best?
Something that won't break and leak right?


Hows this one?:
http://www.google.com/pr...G7Aw&ved=0CHMQ8wIwBA

Awesome

Working with a separatory funnel is awesome! Much easier than pippette, but yeah as you know, you have to work with A/B, because you cant use sep funnel with STBs (the solids would block the stop cock).

Im not sure the material in the sep funnel you quoted, be sure to check out chemical resistant to the solvents, acids and bases you will use. Personally i like working with glass sep funnels (and some resistant stopcock material like PTFE or something).

Also if you plan on doing a bit bigger extractions, maybe better get a liter separatory funnel, 250ml isnt too big.

Awesome, good to hear from ya endless, been awhile.

So would you say A/B is the best way of getting top yields and white spice over the STB? The STB is just easier right?

Also is there anything more on point than the DMT handbook? Like say theres only 500 grams of bark, is there a new tek or a good one that has flexable adjustments when it comes to measuring, so say if a big extraction is done, or a small extraction,
will all the amounts be the same per tek?

Cause i think i read something like 1 gram of bark to 1 gram lye, 5 parts water or something,

what is the correct ratio, or adjustable measurements, tnx

Yeah nice to see you back here! What you've been up to?

Both A/B and STB should get similar yields in my experience. Maybe STBs crystallization can sometimes be yellower, but nothing a recrystallization or two wont take care of. If you're doing an A/B, remember the first acid step will limit everything else in terms of yield, so lets say if you did just one acid boil/soak, it will only get part of the alkaloids, so no matter how many pulls you do later you wont get a large yield. So personally I like doing 4x 40 min (or so) boils on powdered bark (or 3x boils and one long soak, or something), to be thorough. If you dont have your bark powdered, do blend it as thin as possible to increase yields.

I like working with A/B because I can work with the separatory funnel, and also less emulsions, and its less troublesome to discard a liquid than a highly caustic splashing thick soup. But STBs will be just fine if thats what you want

I would do a 500g extraction (or at least 250g) all at once, reduce it to 800ml and use the 1l sep funnel, and fill the rest with the solvent, doing many pulls. All teks on our wiki should work fine, lately I prefer working with limonene as solvent so I do something like BLAB (except doing an A/B and not just an acid soak and then adding base without filtering). Just find what suits your situation the best.

Let us know how it went!

What's your opinion on lime teks where the vinegar is simply mixed into the mush?
I mean to be as thorough as possible with the acid step.

im not sure if I understand your question. What do you mean with being thorough with lime tek? With the lime teks you wont filter out the solids, you keep them when you add the base, so the thoroughness I guess will come from mixing the base with the mimosa well enough, mixing each pull well enough and doing enough pulls.

I think one good general recommendation is to make a long and well mixed last pull, and salt out (or evap/freeze precip if extracting with petrochem-based solvents) separately. Then you can see how many alkaloids there are in the last pull, and if there's still some significant amount coming out, do more pulls.

I made the mistake of buying a 250 ml separation funnel the first time around. Then I bought a 500 ml and that is a tad bit small when processing 100 grams of bark. I found that i had to be sure to reduce my acidifed solution sufficiently to keep the volume such that I could process it in my 500 ml funnel. On top of that I used fairly concentrated lye solution to basify and keep the volume low enough to fit in the funnel along with the NP solvent.

I prefer all glass funnels including the stop cock. I very lightly coat the stop cock with silicon grease. I almost had one freeze up mid extraction. I got it free, let it drain, and took the glass valve apart and lightly coated it. I never had a problem after that.