Mydriasis wrote:Wow infund pulled off a 7%? I'd certainly love to see the technique he used? Link?
The biggest variable I can see is potency of seeds, and if there are seeds with 10%-15% then who is selling them? Or are they not available to the public and strictly used for the manufacture of dye-stuffs?
This was done as an experiment with something else in mind (which I still haven't managed to write down and post) and the yield was 6.4%. I was also quite surprised by this yield. You don't expect to extract 200g of rue and find yourself with almost 12g of harmalas!. The method went like this:
1. Soak seeds o/n in water made pH 4 with acetic acid
2. Soft boil 6 x 1 hour, in each boil the pH was brought down to 4.
3. reduce all boils to a manageable volume.
4. Bring pH to 12 with sodium hydroxide + let the alkaloids precipitate o/n or longer
5. repeat till supernatant is clear (it took maybe 10 times?)
6. dissolve alks in pH 4 acetic acid, then filter them; discard filtrates
7. Add sodium chloride to 10% and manske them
8. Repeat manske 2 more times
9. dissolve alks in water, then add sodium hydroxide to pH 12; let them precipitate o/n
10. repeat step 9 2 more times
11. Take out and dry.
Result was off-white powder. This is pretty much the same strategy as gibrans caapi extraction. The same tek on caapi gave 1% yield though, which is a fine but not wow! yield so I don't think there's something magical in my approach. The seeds were just super super charged.
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