analytical chemist
   
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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bottom of a half-pint jar benzyme attached the following image(s):  xtract.jpg (268kb) downloaded 260 time(s)."Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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x-namwodahs
Posts: 528 Joined: 12-Nov-2009 Last visit: 28-May-2023
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bioassay? They don't think it be like it is, but it do.
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DMT-Nexus member
Posts: 752 Joined: 19-Mar-2009 Last visit: 15-Jun-2019 Location: green heart of caribou
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DMT-Nexus member
Posts: 681 Joined: 11-Sep-2010 Last visit: 24-Dec-2011
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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Shadowman-x wrote:bioassay? none yet. opted to do lc-mc analysis on it first "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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hmm...base peak is 263 m/z, and broad (sample too concentrated) very odd  no idea what that is. this will take some sleuthing "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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DMT-Nexus member
Posts: 12340 Joined: 12-Nov-2008 Last visit: 02-Apr-2023 Location: pacific
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be careful..ive played with grass and instead of DMT, I had weird alks that made me sick..tryptamine feeling with mild visuals but whateer else was there was not nice. Long live the unwoke.
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DMT-Nexus member
Posts: 937 Joined: 23-Oct-2009 Last visit: 25-Mar-2012 Location: Netherlands
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what took you so long? ive been waiting for ages for one of you to do this...  Hopefully one day i will try dmt, extracted by me from the grasses that i will grow myself... Till that day i will be exploring mesc  βThe most important thing in illness is never to lose heart.β -Nikolai Lenin
I know that you believe you understand what you think I said, but I'm not sure you realize that what you heard is not what I meant.
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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fractal enchantment wrote:be careful..ive played with grass and instead of DMT, I had weird alks that made me sick..tryptamine feeling with mild visuals but whateer else was there was not nice. duly noted i'll work on a simple sep tek once I can identify the alks present "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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Dr Do Little
Posts: 187 Joined: 15-May-2008 Last visit: 18-Jun-2015 Location: Underwater
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can't wait to hear the results, then will get SWIMs new patch growing. The hardest thing of all is to find a black cat in a dark room, especially if there is no cat.
-Confucius
"Under the skin of our lives; somewhere deep and early, forgotten, we all share the same dreams"
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DMT-Nexus member
Posts: 4733 Joined: 30-May-2008 Last visit: 13-Jan-2019 Location: inside moon caverns
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Very nice to see this kind of progress, greatly appreciated 
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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if you orders these seeds, whatever you do...don't throw them outside in the soil this is a very invasive species "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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analytical chemist
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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so, how it was done: -p. brach seeds were grown in potting mix with coir and 100 mL 10 mM tryptamine hcl solution added. -dried grass was sonicated for 5 mins in 45 mL household vinegar (pH ~ 2.6), -the slurry was frozen, thawed, sonicated again. -Vac filtration, and the acidic aqueous phase was saved. this was basified with 6 N NaOH up to a pH of 12.56. -added approx 10 mL methylene chloride (Me2Cl2) to the basic phase, inverting the sep funnel several times -poured off the Me2Cl2 in an 8 oz jam jar and evap'ed at RT. (notes: simmering the plant material slurry for an hour or more would work as effectively as sonication. heating up the acidic phase will catalyze [hydro]lysis on the plant cell walls. warm naptha may be subbed for Me2Cl2, do at least 3 pulls.) after transferring a bit into a microcentrifuge tube with a toothpick, swim would estimate around 10 mg is on the bottom of a jar. he wanted to demonstrate that this is an alk-rich alternative to traditional sources. "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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DMT-Nexus member
Posts: 2147 Joined: 09-May-2009 Last visit: 28-Oct-2024 Location: the shire, England
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Really great work man, fantastic! Await the results with much interest.
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DMT-Nexus member
Posts: 14191 Joined: 19-Feb-2008 Last visit: 28-Nov-2024 Location: Jungle
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so what can be the 263 peak, still no idea?
all advances in this area are very appreciated, thanks for the work benz!
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