Anybody have any links on how to run a chromatography development on spice?

What would a good combo be? Water and Bestine maybe? The water would grab all the N-Oxides, etc., and the Bestine would grab the Spice?

Also, a stupid question, why can't one just add acetic acid slowly and directly into the initial (STB) naphtha pull and cause precipitation that way?? (as opposed to freezing it or salting it with some water/mixture) as with Phlux bufotenin extraction... It there some chemical that would do this?

And as for the chromtography... I'm just wondering how to run a simple column on DMT, someone out there must know... Please! Just please tell me what eluents use (amd try to make them easily accessible ones, (i.e., Naphtha, Acetone, etc.). And give me measurements if you can too please. SWIM has never tried a column and would very much like to, because he noticed, even when he leaves the original pulls in the seperatory funnell, nearly microscopic levels do precipitate at the bottom...

SWIM has never worked stoned before, and must say (though he knows he should be extra careful) it definitely adds to the fun!

If he's forced to free perciptate, any suggestions on some ""Flocculent" or whatever its called, to help the crytals gather together...

And what's this about "gassing" it out? I have a chromatograph I could pump butane into or something if someone knows how to do something lieke that!

Hope to hear!

Not much of a chemist, but I believe the reason you don't salt out of naptha is that it's so easy to get the freebase. DMT-acetate is kind of a pain, since it's so gooey, unless you're going for the heat conversion.

Thanks Ragbar...

Any sweet dudes you know on here might not mind being hit up with chroma questions. I just need to know what eluents to use, as well as in what proportions to the freeze precipitated extract pulled earlier. I don't have access to the academic journals right now, but it seems to me there must be regularly available substitutes for chloroform and ethyl acetate and all that. Even if there isn't, SWIM is willing to pull the trigger on buying some of those expensive reagents, chroma seems so interesting, seperating things by fractions and leaching and stuff... I'm just amazed there isn't something you can throw in your initial naptha pulls that would make the DMT fall right out without having to freeze it or pull it into some amount of acidified water...

Anybody? Sorry to be impatient, but SWIM would really like to try some chromatography out: naphtha/heptane? What do you think?

With regards to your not having to put it in acidified water?

The FASA method works just fine... Fumaric acid + anhydrous acetone yields no solubility for the salt.

Unless of course you have some percentage of water on your solvent, which by the way many companies do.

FASA likely yields excessive fumaric acid salts.
has anyone done a m.p. test on the fumarate salt?

btw... EtOAc:MeOH 2:1 is typically the binary mixture used to separate dmt from other simple tryptamines. 70-230 mesh silica should suffice.

there is a nice phototut on indestructables.

Because acetic acid, glacial acetic acid is highly hygroscgopic, it will dissolve the DMT-Acetate whether you want it or not.

Get proper glassware and use the HCL gas method, maybe that will yield good results.