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Making Woodlover - liquid cultures Options
 
PlantPipeline
#1 Posted : 12/8/2020 2:39:48 PM

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Hello Smile

I am currently working with the woodlover mushroom species and would like to create a liquid culture to increase my spawn.
In a Tek on another forum, it is explained, that one can make a nutrition-medium, by soaking hardwood chips 24 hours and filtering afterwards. Now, to sterilise the liquid it says one should "nuke it in the microwave for two minutes" - As I don`t have a microwave, I wonder, if I should boil my mixture instead and if, how long? Or may it destroy the nutritions from the wood in my solution?

Does someone has experience and could point me in the right direction?
It would be really appreciatedSmile Smile

 

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downwardsfromzero
#2 Posted : 3/22/2023 8:32:41 PM

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Shame this question didn't attract a reply sooner. Maybe it will now. If I'd used liquid culture for woodlovers I'd happily answer more definitively. Did you get anywhere with this? Maybe just buy a microwave if you haven't already?




“There is a way of manipulating matter and energy so as to produce what modern scientists call 'a field of force'. The field acts on the observer and puts him in a privileged position vis-à-vis the universe. From this position he has access to the realities which are ordinarily hidden from us by time and space, matter and energy. This is what we call the Great Work."
― Jacques Bergier, quoting Fulcanelli
 
Jacubey
#3 Posted : 3/22/2023 8:35:58 PM
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I've made LCs for oyster mushrooms (wood loving species). I just used the same stuff I used for dung lovers and it worked out fine. I use Light Malt Extract, and I'm fairly sure the sugars in it work for making pretty much any culture.

Consider that all bulk grown mushrooms consume grain spawn at some point. Light Malt Extract is just an extract from another grain, so it stands to reason that: If your mushroom ever uses grain spawn, a grain based LC would suffice.
 
downwardsfromzero
#4 Posted : 3/22/2023 9:36:24 PM

Boundary condition

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Jacubey wrote:
I've made LCs for oyster mushrooms (wood loving species). I just used the same stuff I used for dung lovers and it worked out fine. I use Light Malt Extract, and I'm fairly sure the sugars in it work for making pretty much any culture.

Consider that all bulk grown mushrooms consume grain spawn at some point. Light Malt Extract is just an extract from another grain, so it stands to reason that: If your mushroom ever uses grain spawn, a grain based LC would suffice.

Thanks for the observation, OP question was rather more about sterilizing time of a woodchip tea with boiling water due to lack of a µ-wave though!

To that end a pressure cooker would be as useful an investment as a microwave.




“There is a way of manipulating matter and energy so as to produce what modern scientists call 'a field of force'. The field acts on the observer and puts him in a privileged position vis-à-vis the universe. From this position he has access to the realities which are ordinarily hidden from us by time and space, matter and energy. This is what we call the Great Work."
― Jacques Bergier, quoting Fulcanelli
 
 
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