Here goes. I've failed a couple of times on my grows, learning a lot each time. Right now, I'm doing two more pp5 sort of Annie style grows. One brown rice(Colombian rusts spore), one classic brf/verm(matchapek). The brown rice turned out too hydrated I think, and I've seen this before in my previous rye berry grow. Too much water and then myc piss, then stall.

Could I do a grain to grain transfer on the Columbian so that I get a chance to have dryer substrate and more successfully grow? Or should I just wait it out? We're 3 weeks into colonization (2 for the brf ones). Here are some pics, showing some containers with brown rice that have more/ less time to dry. All were done With the exact same procedure same still AirFlo box etc.

Thanks! I will share some dmt adventures soon too.

Maybe shake?

Works for me, when that happens. Seems pretty typical for MS inocs...and you'd probably have to do it to get a reliable spawn for G2G anyway, so...I say, bang hard!

[Others might vehemently disagree tho...]

Hi there

It looks like you have what is called, wet spot, its a bacteria infection caused by to much water, when the grains get a milky look to them with milky fluid in between the grains, its pretty much guaranteed to be wetspot.

the good news in cubensis mycelium can beat and eat wet spot infected grains, if they some how loose some water.

you could do a grain to grain, I would scrape the fresh mycelium from the top in a glove box with a fork and just use that in a new jar of rye grain, just avoiding getting any nasty bacterian in your new sub. Rye grain gives best results imho, I don't use anything else.

I dunno what tek you are doing .... I believe that the traditional PF Tek or rye grains > bulk sub is the most used method imho.

Ff you do a grain scrape to a new dryer sub strait you should be good.

hope this helps

much love and warmest Regards.

bacteria.

dont shake PP5's
they are not solid
and the lids arent perfeect,
so they suck in micro air drafts when shaken...........

Thanks for all the advice guys. I had like six of these, I shook one, scraped one, and left a couple of them be. Two of the six (the most colonized) came through and 1 of 2 is pinning now. The shaken one died, goopy and foul. The scraped one dried up to much and never colonized new substrated (brf + verm).

I've got a few other grows going, so even if none eventually survive, I'll still have some samples.

Again, thanks!

Those definitely look bacterial, but I'm thinking you are indeed overhydrating your grains as you said.
I've had the exact same problems with slow colonization and stalling jars in the past where there was quite a lot of visible moisture in my jars.

My problems were:
1) Steam evaporation after boiling the rye berries didn't work properly for me. I loaded the jars with damp moist grain thinking this was ok. You definitely want your grain properly swollen and hydrated but DRY on the outside.
2) I didn't use a canning rack in my pressure cooker, and water splashed through the filters of my jars, messing up the water content causing them to come out of the PC wetter than they went in..

As long as you make sure your grain is properly hydrated on the inside yet dry on the outside everything should be a-ok