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Mimosa pudica: Source of 5-MeO-DMT? Options
 
AcaciaConfusedYah
#1 Posted : 10/22/2014 6:20:38 PM

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Hi folks!

According to trout's notes, Mimosa pudica allegedly contains 5-MeO-DMT in the leaves.

My pudica is beginning to drop her leaves, so I've been collecting them. I am going to attempt to collect at least 50grams of leaf material and attempt an extraction using cyb's max ion with a mini A/B at the end.

Does anyone have experience with extracting 5-MeO-DMT from mimosa pudica leaves? If not, hopefully my attempts will be able to shed some light for all of us.
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AcaciaConfusedYah
#2 Posted : 10/29/2014 12:48:04 PM

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Moving along with my quest, my large pudica was trimmed.

Seed pods were collected and then I cut the trimmings into small sections to dry. I'll check back in after drying for total dry weight before extractions begin.

I will be using both limbs and leafs in the extraction
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Sometimes it's good for a change. Other times it isn't.
 
expandaneum
#3 Posted : 10/29/2014 1:17:42 PM

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pudica also contains mimosine which is a toxic compound.
wiki

my advice
read more on the other alkaloids and possible toxins in mimosa pudica before going any further than extracting.

start here and scroll down to the pudica part.
mimosa thread

hope this helps and keep us posted.
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MultiDimensionalTherapy
#4 Posted : 10/29/2014 1:47:40 PM

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i once lived in a place with hundreds of them everywhere! but i didnt had the courage to try an xtraction because of this compounds.
if the chem experts could bring us some light how to separate it, would be really interesting!
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AcaciaConfusedYah
#5 Posted : 10/29/2014 2:30:56 PM

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I was discussing this with endlessness. He said that it looks as though the toxic compounds aren't soluble in nps. I was going to send him a sample for testing before consumption. I'm very curious what we'll find
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Auxin
#6 Posted : 10/29/2014 7:19:18 PM

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Bromcresol green indicates acid at pH 3.8, so thats roughly the point at which it would conform to the following solubilities. More acid or more base and it would be more soluble in water and less in alcohols.
Dont drink a simple brew.
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AcaciaConfusedYah
#7 Posted : 10/29/2014 8:01:17 PM

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Thanks for the pic with info. I had not planned on brewing with it. I'm more of a Parma guy anyways.

I plan on performing a typical a/b extraction with nps as the solvent. I don't know what to expect, if I should expect anything, but we'll see what happens.

I'm more interested in seeing the results than a finished product. I have finished product and mimosa bark. This is purely for science/curiosity
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corpus callosum
#8 Posted : 10/29/2014 8:14:04 PM

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Voogelbreinders epic 'Garden of Eden' references Trouts work by mentioning possible 5 Meo DMT presence in aerial parts, roots of seedlings and also in 15 month old plants harvested in November, with most being found in the roots of plants of this age.

If this is accurate then its only a few days before its potentially worth reaping a harvest of M Pudica.......
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AcaciaConfusedYah
#9 Posted : 10/29/2014 8:29:13 PM

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Mine just so happens to be about 15 months old and plenty of seeds to start again..... But I'm trying leaves and stems that needed to be trimmed first. If proven successful, and this works, I will be a happy boy and begin germinating mama's seeds for next year. She produced a couple thousand seeds. I've collected probably 500.

If it doesn't work, and I make toxic goo - I'll still keep the mom alive because I love that plant.... But I probably won't germinate many of her seeds.
Sometimes it's good for a change. Other times it isn't.
 
MultiDimensionalTherapy
#10 Posted : 10/30/2014 1:50:28 PM

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keep us updated!
Healing someone is an act of love, but how can you love someone whitout loving yourself first?
 
AcaciaConfusedYah
#11 Posted : 11/1/2014 1:39:03 PM

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Extraction begins today.

Dry weight - 49 grams

Dry material has been ground with a coffee grinder and placed in a jar with a small amount of water for one freeze/thaw


EDIT:
1:14pm jar has been thawed and placed in heat bath. Acid added.

5:00pm Basified. The mix changed colors from light brown to dark brown/pink as seen during other bark extractions.

6:30 NPS added and jar shaken
1st lesson learned.... there seemed to be a higher emulsion of nps when shaken than when mimosa hostilis is shaken - yes I usually shake the jar good and hard instead of rolling when working with mimosa. It usually allows for bigger pulls - also inviting more plant fats, but when doing a mini a/b with pulls, that doesn't matter too much.

On a side not, the first pull seems to be quite yellow with plant oils, much like many of my first pulls. I'll do 3 large pulls and then do a mini a/b to the three pulls to remove potential unwanted fats.
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AcaciaConfusedYah
#12 Posted : 11/2/2014 12:27:38 AM

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mmmkk

collected pulls, did the mini a/b, collect new pulls. wait.... tomorrow we might know something. even if it fails, we'll know something. But, dammit, let's hope it works.... visually, it looks like it should work - comparing to mimosa hostilis and acacia confusa extractions


OR.... toxic goo for a confused acacian... yah? mmmm toxic... yum yum yum yum
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expandaneum
#13 Posted : 11/2/2014 4:41:05 PM

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great keep up the good work Thumbs up
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AcaciaConfusedYah
#14 Posted : 11/2/2014 8:19:54 PM

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Sad to say that it appears that there was very little crystals that precipitated. I'm not finished yet, but it does not look favorable
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endlessness
#15 Posted : 11/2/2014 8:26:16 PM

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Do you have any reagent such as ehrlich or whatever else to at least get a first idea on what could be there?

Also remember oils can be misleading in terms of weight, seems less than it is. And even if its not much but its a clean profile of wanted alkaloids, it would still be great Smile
 
AcaciaConfusedYah
#16 Posted : 11/2/2014 9:43:30 PM

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Hey Endlessness!

I do not have any reagents, but I'll begin to look for a source.

I'm also keeping in mind that I did not use the roots. As seen in other species, the roots seem to contain more of our favored alkaloids - though trout mentioned he suspected it in the leaves.
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endlessness
#17 Posted : 11/2/2014 9:52:42 PM

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https://www.dmt-nexus.me...aspx?g=posts&t=25771

Here you have some reagent suppliers.

Good luck and keep us updated!
 
Pharmacognosis
#18 Posted : 11/12/2014 10:03:59 AM

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Mod wrote:
No discussion of buying/selling except for live plants/seeds. Please read the Attitude.
Great work so far AcaciaConfusedYah and endlessness, hope others pursue this avenue of research as well.

 
AcaciaConfusedYah
#19 Posted : 11/12/2014 4:09:17 PM

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I haven't gotten the reagents to test - money is short at the moment.

I did yield a nice yellow goo. Though it was not a lot, I am hopeful that it contains the things we are looking for. I have one more pull(the middle pull) that I haven't precipitated yet. Maybe the middle will show me some crystals
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AcaciaConfusedYah
#20 Posted : 12/9/2014 12:55:41 AM

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So, unsatisfied with my first attempt, I am attempting another extraction.

This time, only using 15 grams of dried leaves and dried limb BARK only. On the first extraction, I included the woody material of the small limbs in the total weight. In this test, I am not using the woody part of the limb.

Also, I am taking a different approach to the extraction. I am performing a "DRY" Style tek with IPA 91% as solvent number one. After the IPA is evaped, I'll dissolve the evaporated remains into heptane and then do a mini defat, and do a final pull again with heptane.

we'll see.... Smile
Sometimes it's good for a change. Other times it isn't.
 
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