Instead of the traditional np wash, then polar extraction, i had a few ideas as far as extracting lsa from the seeds w/o the body-load/nausea inducing toxic compounds, and at relative purity.
To be more specific i was thinking of either slightly acidified dh20 (tartaric acid ph6 maybe?), acidified isopropyl (or not acidified isopropyl), or a combination of iso/acidified dh20 with ground seeds.
Pulling 3-5 times over a week, letting it soak for a fairly good amount of time (1-2 days per pull). A n2 atmosphere would be put atop this solution to prevent degradation of the alkaloids via wine preserver spray. Also the container would be kept away from heat or light during the process.
If using just acidified water, then a heptane defat/emulsification would be done 3x on the filtered water, then the water would be separated, reduced, and made into a tincture, or evaporated and washed again with heptane, then stored as a powder or redissolved in dh2o to make a tincture.
If using alcohol/acidic water, evap the pulls away from any heat or light, preferably under vacuum with a o2 devoid atmosphere. Wash the dried crude extract with heptane 3x, then redissolve in dh2o, filter, and either stop there as a tincture, or evap and wash with heptane again to ensure all the np soluble stuff is removed. Then redissolve in minimal dh20 and store in freezer, or store powder in freezer.
Basically i want to get as pure as i can w/out an acid base, and using the least amount of NP solvents as possible. Why wash with large amounts of NP first, when you can just use much less to clean up a much smaller amount of crude extract later.
I recently did a small scale extraction on 100 RC seeds, and washed with heptane 3x, then pulled with iso 3x. Evaporated the iso, then washed again with heptane. What was left in the dish was redissolved in dh2o, then evaporated. Upon drying, and attempts to scrape, the product was crystalline, and "sprayed" when attempted to be scraped up with a razor blade. I did not attempt to do this as it was spraying all over the place, and just redissolved it in dh2o and froze it as is. Crystals were very bitter and most definitely active though. As is the tincture.
BTW, for anyone who ends up with goop from a NP wash, then alcohol extraction, consider washing your end goop with NP to remove extra crap. It worked wonders for me. Went from yellow goop to crystals when recrystallized w/dh2o after washing the goop. Seems the alcohol pulls crap that NP's wash away, even if you did a thorough NP wash before hand.
I like how the small scale extraction worked out, but would like to try something that uses less NP solvent, and is relatively easier compared to the traditional extraction.
Any ideas on how this might work out or advice on how to go about this? Is the acid necessary at all?
Which solvent would be ideal here? (iso, dh2o, acidified dh2o, acidified iso, or acidified iso dh2o mix?)
I have some hbwr and RC seeds on the way and want to extract them or freeze them asap to prevent any degradation, and want to have a sound tek planned out before i attempt anything with them.
Any tips, advice, or comments is appreciated-
Thanks
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