DMT-Nexus member
 
Posts: 2291 Joined: 26-Mar-2008 Last visit: 12-Jan-2020 Location: The Thunderbolt Pagoda
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Let's say SWIM had lost a 20g or so to Rhizopus, due to improper drying techniques. Would it be safe to perform ethanol extraction? SWIM understands that this mold is only dangerous if aloud to sporulate within the body. SWIM doesn't believe that the mold or spores could survive ethanol extraction. The ultimate goal of the extraction is to yield crystalline psilocybe. The cubes are already soaking in ethanol, but SWIM wants to make sure that there's no toxins that would end up in the final product.
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analytical chemist
 
Posts: 7463 Joined: 21-May-2008 Last visit: 03-Mar-2024 Location: the lab
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do an a/b extraction one of the perks of extractions, biological contaminants aren't really a factor. strong base followed by nonpolar solvent extraction...yeah...don't worry about it. in the end, you'll pull alkaloids, not mold/spores (the latter will likely be in a thin layer between the aqueous and nonpolar phases) "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
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Kalt und Heiß, Schwarz und Rot, Kürper und Geist, Liebe und Chaos
Posts: 4661 Joined: 02-Jun-2008 Last visit: 30-Apr-2022
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...unless the Rhizopus fungus has some toxic alkaloids in its mycelium. Depending on the nature of these alkaloids, they may come along with the mushrooms alkaloids in an A/B extraction. But yeah, it is very unlikely, SWIM's FOAF would go ahead, do the extraction and try the product. Need to calculate between salts and freebases? Click here! Need to calculate freebase or salt percentage at a given pH? Click here!
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DMT-Nexus member
Posts: 5826 Joined: 09-Jun-2008 Last visit: 08-Sep-2010 Location: USA
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If you use a good quality filter paper, the spores will be filtered out for sure. The biological organisms won't survive being in the solvents and the pH swing from low to high will also kill most microorganisms. Some microorganisms produce extremely toxic alkaloids. That’s what I’d be afraid of. The alkaloids from the microorganisms could make it through the process, and if toxic, could possibly kill you. For that reason, make sure you can crystallize and identify the alkaloids your attempting to extract. I'd recommend throwing them out. Your health is more important than anything else. You may remember me as 69Ron. I was suspended years ago for selling bunk products under false pretenses. I try to sneak back from time to time under different names, but unfortunately, the moderators of the DMT-Nexus are infinitely smarter than I am.
If you see me at the waterpark, please say hello. I'll be the delusional 50 something in the American flag Speedo, oiling up his monster guns while responding to imaginary requests for selfies from invisible teenage girls.
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DMT-Nexus member
Posts: 686 Joined: 29-Jul-2008 Last visit: 19-Sep-2013 Location: Western Spiral arm of the Galaxy
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has anyone ever attempted an A/B extraction with mushrooms? it would be interesting to get some measures. i would guess that the process won't be the same as with mimosa or would it? which solvent would be used to pull? I am the Lorax. I speak for the trees. I speak for the trees, for the trees have no tongues. And I'm asking you, sir, at the top if my lungs.. (all posts are fictional and are intended for entertainment purpose only)
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Kalt und Heiß, Schwarz und Rot, Kürper und Geist, Liebe und Chaos
Posts: 4661 Joined: 02-Jun-2008 Last visit: 30-Apr-2022
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Very similar indeed, yet one has to be more careful with pH extremes (pH of 4 is more than enough for acid extraction, whereas pH>11 should be avoided for the basification step). The choice of non-polar solvent is also critical; naphtha wouldn't work very well and freebased psilocin is water soluble (xlogP=1.6). DCM reportedly would work fine, while SWIM's FOAF has used isobutanol for the said purposes success recently. Need to calculate between salts and freebases? Click here! Need to calculate freebase or salt percentage at a given pH? Click here!
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DMT-Nexus member
Posts: 686 Joined: 29-Jul-2008 Last visit: 19-Sep-2013 Location: Western Spiral arm of the Galaxy
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could one also do an STB with it? I am the Lorax. I speak for the trees. I speak for the trees, for the trees have no tongues. And I'm asking you, sir, at the top if my lungs.. (all posts are fictional and are intended for entertainment purpose only)
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DMT-Nexus member
Posts: 2291 Joined: 26-Mar-2008 Last visit: 12-Jan-2020 Location: The Thunderbolt Pagoda
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Apparently Rhizopus oryzae contains an ergot alkaloid called agroclavine, and agroclavine is toxic. So SWIM'll be trashing the batch.
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