My guess is that all of the alkaloid salts are extracted even before then. I only did 3 boils for my extractions, and the water was nearly colorless (but not clear) with the third boil. Acetate salts of harmala alkaloids seem to be fairly soluble in hot water, so after the third boil there’s probably very little left.
(As an aside, it’s also a myth that MHRB powder needs to be boiled to get DMT of it. I recently tried a room temperature extraction – room temp. water plus a splash of vinegar, stir, settle, decant x4 – and my yield was just as high as using boiling water. So for all you A/B people out there using MHRB powder – boiling is not necessary!)
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