I would like someone with more chemical knowledge to chime in here, but from what I understood gramine IS soluble in acidified water, so you couldnt clean it that way. The gramine subject comes up time and again and so far with no real conclusions or reliable tests done by someone of the community. In any case unless I missed something, the most promissing way of separating dmt from gramine seemed to be through heptane or naphtha recrystalization, because gramine has a lower xlogp than DMT.
I get conflicting informations about gramine's xlogp though, if I understand correctly chemspider seem to say logp is 1.9, which would be trickily close to DMT's 2.0, but other sources say its 1.3 or 1.8, so im not sure. Can any of the chem members answer this one?
Another possible way seems to be through the melting point. Gramine's boiling point is around 300 degrees celcius, so if one used lower temperatures closer to 150 degrees, one could possibly vaporize only dmt and not gramine.
If I were to bioassay a phalaris/arundo/phragmites extraction, I would only do it both after a naphtha/hexane/heptane recrystalization, and vaporizing with lower temperatures, just in case
