Once I tried to do a montotub without any of the double row air exchange stuffed holes. Things seemed ok at first, but the micelium stalled half way through. I gave it extra time out of curiosity, but it had just stopped.
The way I understand it is that micelium needs to breathe O2 just like us, and more micelium needs to breathe more. This means enough FAE which depends of the micelium volume.
Pre-made designs are made so there is enough O2. Home made/new designs may work one day, but if you add more micelium mass, issues due to not enough FAE may show up.
It's like a bunch of people stuck in a tight space with little FAE. One or two people may survive, but keep on adding people and at one point they will start dying. So again, you have a max biomass for a certain FAE. In the poor monotub FAE experiment above I think the micelium grew as big as it could and then understood that it had to stop due to the poor FAE.
Now I put monotubs atop of plants so the CO2 the micelium exhales cascades on them, just for fun.
Oh, and as you all may know maximizing O2 may not be ideal either. Some claim that to fruit, lower O2 levels give the best result. That could be why the momotub is so sweet: Fixed FAE through stuffed holes means that as colonization reaches 100% O2 levels drop just in time for fruiting. You can get different size fruits as the mushrooms reach up to find more O2.
These are just my musings, not facts. One day I may get an O2/CO2 sensor to try to observe this effect. Not just for curiosity, folks publish ideal CO2 levels for fruiting, so using g the sensor as feedback the number of FAE holes can be adjusted so by the time colonization is 100% the CO2 level is ideal. And this optimization should be for a fixed substrate mass.
All this is hypothetical until it can be tested with the O2/CO2 sensor.