I just happened to obtain some Blue_Morning_Glory_Roots, and some flowers as well.
I plan to extract via A/B, when dried, unless i find a better method, or if you know one.
Found something interesting:
ergotish alkaloids in the morning glorySpecial thanks to our User 18517, who originally quoted this on the shroomery! and for the clandestine help here
from Rätsch's encyclopedia:
The leaves contain the ergot alkaloids (ergoline derivatives) ergometrine, isolysergic acid amide, and lysergic acid amide (LSA). The biosynthesis of these indole alkaloids occurs in the leaves, while the subsequent translocalization leads to an accumulation of the alkaloids in the seeds.
Quote:
Hooty said:
right on, well then the post came from a member of the hive (https://www.the-hive.ws) who went by the screen name halfapint. The original post was from 07-12-00 and I'll post it and some other posts from the thread which are informative that were made by halfapint. I'll list the date of the post before each post to kind of seperate them.
from
https://www.the-hive.ws/...;sb=&part=1&vc=1 07-12-00
Morning Glory Whole Plant Extract
Who says submerged culture of Claviceps is preferable to exploiting profuse native morning glories to get lysergic goodies?
SWIM wanted to check the morning glories of her native environment, on a hunch. The hunch came from persistent reports of allergy / toxicity at the touch of morning glory foliage, in Mesoamerica and in North America. Large effects from trace amounts is a lysergic specialty, and mg's don't have a bunch of other bad alkaloids in them. Everybody says morning glory seeds only, and then just from certain varieties.
So thinking such thoughts, SWIM hopped out and gathered up a bunch of wild plants, genus ipomoea. She performed extraction procedures on the first 4 spp. of wild or feral mg's she found, with 2 domestic varieties, blue and purple, for comparison.
So SWIM extracted in this wise: the air-dried plant material was macerated, then moistened with 0.1 N sodium carbonate aq. to free the base. The material was percolated in a column with 1 liter petroleum ether added dropwise over 6 hours. Dilute acid, SWIM said 0.02 N HCl, was used to extract the hopeful-amide from the nonpolar solvent, in three vigorous washings (200, 200, 100 ml) in a 2-liter bottle. Coincidentally, that same bottle was used as separatory funnel, by inverting it and carefully loosening the cap.
But that ain't the point. The point is, that SWIM worked under black light all she could, to see what was going on. She saw florescence in the layers, giving visual feedback on the progress of the separations. The more concentrated the alkaloid, the brighter it glowed, typical of the indole ring alkaloids. In this case, SWIM figured glow meant LAA, lysergic acid amide, because morning glories don't have any appreciable amounts of any other lysergic acid derivatives, nor any other indole alkaloids, nor any other alkaloids at all.
So after the brightly-glowing aqueous fraction was cut out, SWIM made the solution basic with ammonia, giving a flocculent precipitate which took forever to settle out. But it glowed.
SWIM did this on all 6 plant materials, and the situation was the same with all the extracts of morning glory whole plants. Making the same type extraction on crushed domestic seeds did produce a greater concentration of product, as might be expected.
But I says to Swim, I says look. The respectable books and sources and stuff all say it ain't true, you can't get lysergic stuff except from the seeds, and just certain kinds of seeds, and better you just go score Hawaiin Wood Rose and forget plain old morning glories, but you just proved them wrong. Six times out of six tries, you got glowing alkaloids from the leaves, and vines, and stems, and tendrils, and seed cases, and stuff like that all mixed together. So is somebody trying to hide something, or did you just get lucky?
SWIM says, well fuck that, the real reason to use morning glories is because there's only one compound worth worrying about, but if you use ergot you need to fool with seventeen different types of ergotosnotalines.
Well this is a cliff hanger, and SWIM isn't real happy about using ammonia because it makes the ppt. way too puffy and hard to filter for the next stage, but she just persisted with the same technique so all the batches would be treated the same, so she could compare them.
Next time I spot her, I'll ask her how it's going.
Half-a-Pint
Half a pint's a half a pound, a half the world around, around.
07-13-00
Re: Morning Glory Whole Plant Extract
I figure I can't get too specific with specifics. Certain aspects of my personal life may be sensitive to eg. cross referencing species abundance by geographical distribution et al. Dig? Morning glories are endemic to Southern woods, and grow as a predominant weed species in Midwestern cornfields. They grow in disturbed forest lands in North America generally. They grow in ditches. The Southeast has such an abundance of species, they even outnumber Central American species, the genus must be considered indigenous. Go downhill, look for a watercourse, you'll find them. There is even a degenerate red species which has lost its leaves and foliage, and grows on tree bark as a saprophyte. Also introduced species thrive, in all regions, when they get out.
The amount of plant material was ~100 grams in each case, perhaps a bit less because this was dry weight and herbaceous materials lose a lot of weight when they dry. Yes there were differences between the species, but as I mentioned I don't expect to provide this particular bit of documentation.
Just call me grumpy,
Half-a-Pint
Half a pint's a half a pound, a half the world around, around.
08-09-00
Re: Morning Glory Whole Plant Extract
OK, got a chance to do a little research in the best and most productive information repository on the planet, the old Hive.
Here is a really and truly Reference to show that my dream (mentioned earlier) was not merely the delerious hallucination of an acid-starved brain.
From the Chemistry Discourse, KrZ posted 02-25-2000 09:07 AM on the old Hive:
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The last reference I have refers to isolation of ergot alkaloids from Ipomoea violoacea:
Moekatis et al, Biochem. Physiol. Pflanzen, 1973, 164, 248.
Seeds:
-pulverized seeds defatted in pet. ether for 5 hrs.
-1 g of this material shaken 3X (each for 1 hr.) w/ 20mL of (2g tartaric acid in 30 mL H2O, 70 mL acetone) mix
-combined extracts heated on H2O bath (55?C) to expell acetone
-tartaric acid soln. shaken 3X w/ anhydrous ether, then basified (pH 8-9) w/ NH4OH
-from this soln' alks extracted 3X w/ 10 mL DCM
-combined extracts reduced to 1 mL and chromatographed
Roots, Leaves, Stems:
-pulverized material (15g) wetted w/ 3% NH4OH
-extracted w/ 200 mL DCM in Soxhlet for 6 hrs
-condensed to 20 mL & shaken 4X each w/ 10mL 2% tartaric acid
-total of 40 mL soln. extracted 3X each w/ 10mL anhydrous ether
-tartaric acid extract made basic w/ NH4OH (pH 8-9) & shaken 3X w/ 10 mL DCM
-total of 30 mL DCM extract condensed to 0.3 mL & chromatographed...
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so there. I know, there's no results of a quant. nature in this brief excerpt, even as I gave no quantities, but the point is qualitative. There are ergotogoodies in m.g. vegetation, and in my experience that is the rule and not the exception.
Otherwise, why would the working pros who did this real published chemistry have bothered to mention extracting from the vegetation, in their peer-reviewed paper? They might have got a wild notion, like SWIM did, and done the legwork, like SWIM did, but if they got zero yields (unlike SWIM), they likely as not would have quietly let it drop when it came time for the writeup.
I haven't seen their paper and don't know their results. But by my guesses into scientist behavior, my bet would go that they found alkaloids also. No point in chromotagraphing 0.3 ml of nothing...
Half-a-Pint
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Half a pint's a half a pound, a half the world around, around.
*ALL WAYS WITH LOVE