normal scan range is 15-2000 amu..
since it's ESI, it can fragment 100,000 amu macromolecules.

I'll get into zoom scan mode later, when looking for specific analytes, and ms/ms when elucidating isomers.

That's great, you really have got yourself a bargain there!

oh believe me, I know.

thanks.

I'm bidding on a Thermo Surveyor MS pump/autosampler/PDA detector stack. If I can get it for 1500 or less, that's another steal.
I already have all the software, cables, and even configurations on the comp. the whole shebang would sell for 25K on ebay, I'd have it for less than 7500.

gettin there.


still baselining.
notice, the range is set to tryptamine, phenethylamine, ergoline, and beta-carboline range.

NL will be around E^ 7 when optimized.

been troubleshooting all day to get all diagnostics to pass, namely the RF modulation and frequency, and optics lens voltage offset. it is now running like a swiss watch.

just need to calibrate and tune. waiting on calibration solution

Well this is very exciting

I've been away far too long! Benzyme this is awesome!

thank you.

I was formally trained in the art of LC-MS/CE-MS, and am at a decent point financially, so I pulled the trigger on a hell of a deal. Just ordered an HPLC module too, same one I used in school. I'll get this all working like a well tuned system, with sensitivity in the femtomole regime, shortly.

Nexus historical moment right here - proud of you Benz.

thank you.

it's...challenging, rewarding, and always a learning experience. I learn something everytime I power it up.
yesterday, I noticed the electrospray plume under low lighting, it was a faintly glowing purple color.



and I put in an order for reserpine standard. with that, I can properly tune this instrument as well as a number of other instruments, and perform a validation. I already have calibration solution, as is depicted in the video.

Exiting,
make sure you keep Beaker in check

BEFORE ITS TOO LATE



Fine equipment though. I still cant decide which direction in chemistry to go

Its hard to find something that I really want.

Will expand to make a huge analytical lab? With even more equipment?

Thats actually not that bad. If you get a good lab going you can have plenty of work and thus even more equipment.

If I do, it would be an FTIR spec. NMR isn't practical in a house.. but as you can see, mass spec is.
maybe I'll lease a space eventually, but I really just want to master mass spec, and do some more method development. perhaps
create some inventions along the way.

I'm still waiting on Alfa to review my account, and send me the reserpine standard. Once I get that, and properly tune, I will report back and begin accepting samples. I look forward to elucidating the efficacy of certain procedures regarding extraction of cubes and the like. I have analyzed that before.

Reserpine arrives tomorrow.

meanwhile, I was able to run an old sample this weekend...

This sample was not reserpine then, since it's mollar mass is 608.68 and that m/z peak is nowhere any integer number of z

Do I make sense somehow or display stupidity here?

you are correct.
the sample is a smaller molecule, and much more... mind-manifesting.

Nice gear! Does this measure also the quantity of a molecule in a sample or only if it's present?

both.

TIC (total ion count) chromatograms can give quantitative information about the relative amounts
in a sample. MS/MS data gives structural information.

That's quite a large peak around m/z 188. Hmmm....

for those unsure of what Reserpine is for...
basically, the ion trap mass spectrometer makes measurements based on resonant frequency, a fixed freq., and is tuned to have highest sensitivity to 609.2 m/z. Reserpine is the tuning standard. after an acceptable level of sensitivity (aka NL, normalized level) is attained, a calibration is performed.