Hello all,

This is my first post on DMT-Nexus, so hello to everyone.

First of all I would like to apologise for my basic knowledge of chemistry and extractions.

SWIM wants to do a Bufotenine extraction from Anadenanthera colubrina. SWIM has found an extraction technique that seems to be a lot simpler and easier than an A/B extraction and from reports seems to produce higher/different yields.

SWIM had not heard of this technique anywhere else and wanted to get some opinions on the chemistry of the extraction and experience if anyone else has tried a similar technique.

SWIM has also read the Jorkest's D-Limonene Bufotenine Extraction on DMT-Nexus Wiki, however found the following extraction to be quite different.

SWIM sourced the procedure from:
http://www.erowid.org/experiences/exp.php?ID=64707

The procedure outlines:

Boil the powdered beans in water made pH 3 with hydrochloric acid for about an hour, and then filter out the beans, and repeat 2 more times with new water, also made pH 3 with hydrochloric acid. Concentrate the combined water extracts down and evaporate to leave some solid brownish gunk. Weigh this gunk and measure out an equal portion of calcium hydroxide (the same pickling lime used to make Yopo and Vilca snuff!).

Dissolve the gunk in a small amount of isopropyl alcohol, just enough to make it a thick syrupy liquid, then add an equal portion of water, then mix in the calcium hydroxide. Mix it very well. You don’t want any clumps. It should be the consistency of thick pea soup. Let it sit for about 6 hours for the calcium hydroxide to react with the bufotenine, creating the basic salt calcium bufotenate. Now evaporate it (you can use an oven at 300 F for this step). Once it’s evaporated completely add a generous portion of acetone. Mix it well. The acetone won’t dissolve any of the calcium hydroxide, or much else, but will easily dissolve the calcium bufotenate. Let it sit an hour or more for the non-soluble particles to sink to the bottom, once the acetone takes on a clear dark amber color, poor your mix through a filter to obtain the acetone.

You can repeat the acetone extraction with new acetone a few more times until its clear. Evaporate the combined acetone to get an extremely potent extract that is nearly 90% pure calcium bufotenine, which is one of the most potent forms of bufotenine. It’s the form found in properly made Yopo and Vilca snuff that has been used for thousands of years in South America. It’s more psychoactive than free-base bufotenine and much more psychoactive than the acidic salt form found in the unprocessed beans.

From previous reading SWIM thought that the first step of boiling the powdered beans would potentially destroy the bufotenin??

Also SWIM does not understand why Calcium Bufotenine would be more potent than free-base Bufotenine??

Thanks everyone for your help, I really appreciate it.

Matty

Bufotenine is very heat stable so boiling in water is not the problem.

Ehm... I prefer the jorkest tek over that one anyday. First of all because you have to be very careful with your pH when adding and hydroxide to bufotenine as it can damage the molecule after a certain pH for a certain amount of time.

Also you'll probably find that for that tek 90% pure is very optimistic.

Ahhh,

Thanks for your reply.

Can anyone explain to me main differences between Calcium Bufotenine and free base Bufotenine?

Cheers,
Matty

Actually, there are strong suspicions that the result product is TOTALLY DIFFERENT from bufotenine. Calcium hydroxide (or more correct, the high pH) and bufotenine seem to react and form a derivative over a couple of hours, and this derivative seem to have effects that strongly differs from those of freebase bufotenine. I strongly suspect this derivative is dehydrobufotenine; the nitrogen atom (with the methyl groups) has formed a covalent bond with position 4 and effectively removed a hydrogen atom. It's not even chemically a tryptamine anymore.

If this is the case, then this is no longer just an extraction of bufotenine. It's a conversion from bufotenine to dehydrobufotenine and then an extraction of this new derivative.

Yeah, I think dehydrobufotenine is being created by the calcium hydroxide. This theory is supported by Alexander Shulgin. Something is definitely happening. SWIM and several other SWIMs have reported tests in which the bufotenine seems to change after mixing it with calcium hydroxide for a few hours.

I’d like to see more work done on this. SWIM has at times got the mix just right and the results were nothing short of amazing.

Thanks for helpful replies, this is very interesting.

The SWIM will experiment with this technique and report further.

Would love to hear from other SWIM's regarding the same or similar technique.

On another note, the SWIM cannot source pickling / food grade lime and was wondering if builders hydrated lime would be safe? It states Calcium Hydroxide on the packet.

Thanks,
Matty

I'd just go with sodium carbonate.

Would this still create Calcium Bufotenine or Dehydrobufotenine as others suggest?

Is there anything unsafe with using builders Calcium Hydroxide rather than food grade?

-Matty

Food grade is better I suppose, but as you filter it out of the final product it should be fine.

Just look at all the folks who use NaOH for DMT extrations... and that stuff's nasty!

I use ph correction for Aquariums for my calcium hydroxide.

Sodium carbonate will not degrade the product because the pH can't get high enough.