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Significantly increasing DMT content of DMT-plants? Options
 
SKA
#1 Posted : 8/12/2009 6:55:04 PM
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SWIM has been wanting to grow his own DMT-source plants, but up North here in The Netherlands this is pretty difficult with most DMT-plants.

So this got SWIm thinking:

Could one perhaps purchase and grow an easily grown DMT-source plant, like Desmanthus Leptobus/Illinoensis or Psychotria Viridis, that grow fast and easily in cold, dark climates and then put effort in increasing the plant's DMT-content?

Maybe this can be done by feeding the plant certain Precursors that it will convert into NN,DMT? Tryptophan as precursor maybe?
And perhaps the plant can be fed or injected something else that stimulates and hypes up the natural BioSysnthesic mechanism that creates DMT to make far more DMT alot faster than usual?

Anyone?
 

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obliguhl
#2 Posted : 8/12/2009 10:14:14 PM

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If I recall correctly, there is one phalaris strain available with heightend alkaloid contents (Big Medicine). So it can be done through breeding processes...or so it seems. I don't really have a clue but I like the way of your thoughts. We have to think about tomorrow were growing dmt plants will be neccessary for our own safety.
 
dread
#3 Posted : 8/12/2009 10:27:13 PM
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I would expect tryptamine to work better than tryptophan. At least this is the case with mushrooms.
 
neuro_rocket
#4 Posted : 8/12/2009 11:30:36 PM

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SWIM knows somebody who is growing P. Brachystachys and has a few very nice plants that are a month old and a few smaller ones that are a few weeks old.

To make them more tryppy SWIMS friend stresses them by not giving them water for a few days and then giving them water laced with alot of nitrogen (he might also give them some tryptophan if he can find it). He also pokes and cuts them with a needle, which is supposed to quadruple their tryptamine content.

When he harvests them he only cuts off the top half of the leaf because that is where the highest concentration of goodies are (trimming also increases tryptamines). He then sticks the trimmings in the freezer until he has enough to extract from.

SWIM strongly encourages the growing one's own DMT source, because if DMT ever does go mainstream the government is probably going to crack down on MHRB.
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SKA
#5 Posted : 10/14/2009 12:40:27 AM
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Is there no way to make the naturally present BioChemical mechanism that creates DMT in DMT plants "work overtime"?
I assume the plant converts a certain chemical, that it drank from it's soil, into another chemical...into a chain of conversions that eventually leads to DMT. I'd like to know more about the chemcicals in the chain conversion that leads to DMT. A google search gave little usefull information. Anyone here who can give me some information about that?
 
dread
#6 Posted : 10/14/2009 12:42:02 AM
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Tryptophan -> tryptamine -> NMT -> DMT.

Tryptophan is a proteinogenic amino acid, so it is something that's readily available for most organisms. Most simple plant alkaloids are biosynthed from amino acids.

Mushroom potency has succesfully been increased by adding tryptamine hcl in the substrate, but of course mushrooms work completely different than plants.

I also hear cactus potency has been increased by injecting them with tyrosine or tyramine (4-hydroxy-phenylalanine or 4-ho-phenethylamine) I forget which of them was used.
 
MagikVenom
#7 Posted : 10/14/2009 1:51:58 AM

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Desmanthus Leptobus/Illinoensis

Both of these will give you DMT levels in the rootbark that are twice as strong as Psychotria Viridis leaves. Psychotria Viridis needs a warm humid environment to thrive and grow rapidly and is extreamly sensitive to cold temps.

Desmanthus Leptobus/Illinoensis however can with stand a good freeze and grow with little care. There is no need to try to make them more potent as the rootbark will kick you ass nicely as it is.

The grasses are all complete bunk in comparison to these two.

Many Desmodium species are also cold/freeze hardy and could be considered, Delosperma is another one to consider for your requirements.

So get some seeds and master growing you plant of choice. Once you can grasps the controls firmly ie by growing successfuly the plant of choice. You will be ready to move on to your experiment of significantly increasing DMT levels.

One step at a time you shall riseSmile

PEACE
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'Coatl
#8 Posted : 10/14/2009 5:16:07 PM

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To start with I think it'd be best to just try and find a few really potent clones of Psychotria viridis, once you have a few clones which are potent, then try breeding them.

This will likely take a long time, but we gotta start somewhere!

Thanks for posting!
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neuro_rocket
#9 Posted : 10/14/2009 6:42:46 PM

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MagikVenom wrote:


The grasses are all complete bunk in comparison to these two.




SWIM has to disagree. He is currently growing P. Brachystachys and D. Illinoensis. The grass has been growing for half as long and he has already collected enough material to try for an extraction and he has many new sprouts.

The Desmanthus takes a fairly long time to grow and is very hard to harvest without killing the whole plant.

Phalaris strains have been developed that yield a considerable amount of DMT and with proper stressing they can yield just as much, if not more than MHRB or Desmanthus, with much less expense than the first and much less effort than the latter.
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SKA
#10 Posted : 10/14/2009 9:54:51 PM
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SWIm wants to invest time in mastering the cultivation of a DMT source plant, but before he does wants to be sure what his best choice would be.

SWIY sais D.Illinoensis grow very slow and are hard to harvest without killing them. So perhaps there are any other worthwhile DMT-source plants that can survive Holland?
Maybe some that grow faster than D.Illinoensis and has DMT in foliage?(so harvesting it doesn't equal killing it)

Would any Acacia's grow here? Even if I protected them from the winterfrost in a thermally insulated greenhouse?
 
'Coatl
#11 Posted : 10/14/2009 11:15:36 PM

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Psychotria makes a great house plant, so you can grow them indoors in a cold climate.
WARNING: DO NOT INGEST ANY BOTANICAL WHICH YOU HAVE NOT FULLY RESEARCHED AND CORRECTLY IDENTIFIED!!!

I am Teotzlcoatl, older cousin of Quetzalcoatl. My most famous physical incarnation was Nezahualcoyotl, but I have taken many forms since the dawn of the cosmos. In this realm I manifest as multiple entities at a single time. I am many, I am numbered. I am few, but more than one. I am a multifaceted being, a winged serpent with many heads. We are Teotzlcoatl.

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neuro_rocket
#12 Posted : 10/15/2009 12:38:14 AM

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Phalaris Brachystachys would also work very well, it grows and spreads fast in most climates (needs light to germinate though).

PM me if you'd like some seeds.
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SKA
#13 Posted : 10/15/2009 1:24:24 AM
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neuro_rocket wrote:
Phalaris Brachystachys would also work very well, it grows and spreads fast in most climates (needs light to germinate though).

PM me if you'd like some seeds.


And what would the average DMT content percentage of Phalaris Brachystachys be?
Wikipedia sais "Aerial parts up to 3% total alkaloids, DMT present[citation needed]", but how much of the 3% alkaloids is DMT isn't said. Anyone?

Also Gramine, a very toxic and harmfull chemical, seems to be present in many Phalaris species. So SWIM hopes that Phalaris Brachystachys is Gramine-free, cause SWIM doesn't feel like going through the hassle of separating the DMT from the Gramine. SWIM is no master Chemist.

He is well familiar and successfull at extracting DMT from MHRB with a very simple yet effective STB method. He also wonders if using Phalaris Brachystachys would require extra defatting steps in the extraction process. This could complicate the extraction process and make it alot more expensive(extra solvents to defat) and SWIM is neither a rich man nor a skilled Chemist.

SWIM understands that, with a thermally insulated greenhouse to protect it from frost and perhaps some extra hours of artifical day light in the dark winter, Phalaris Brachystachys might be able to grow all year round in Holland. In that case he would love some seeds. Or is that being too opsimistic?

Anyone?
 
soulfood
#14 Posted : 10/15/2009 1:34:22 AM

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My understanding is that all phalaris species need a good defatting.
 
balaganist
#15 Posted : 10/15/2009 11:03:33 AM

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I would love to try this but I'd want to know I have a good strain first. Lots of research needs to be done on this still. One day when I have the time I will start experimenting...
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SKA
#16 Posted : 10/15/2009 2:50:19 PM
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'Coatl wrote:
Psychotria makes a great house plant, so you can grow them indoors in a cold climate.


SWIM understands it has DMT in it's foliage so that's a big +.
Does Psychotria Viridis need artificial light and-or extra heating and-or humidifying to survive long, dark and cold winters?

SWIM has read on Wikipedia that Diplopterys Cabrerana(Chaliponga) is a rainforrest vine that contains between ) 0.17 and 1.75%.

If vines grow under the thick, closed shadow of the canopy of the rainforrest, then perhaps they can survive the darkness of northern european winters. Could Diplopterys Cabrerana survive a north European winter in an indoor terrarium, perhaps with some extra heating and humidity?

SWIM knows most rainforrest vines grow rediculously fast. Is this also the case for Diplopterys cabrerana?
SWIM really hopes so.
 
SnozzleBerry
#17 Posted : 10/15/2009 6:00:54 PM

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soulfood wrote:
My understanding is that all phalaris species need a good defatting.

Your understanding is correct. With phalaris the defat is crucial.

SKA wrote:
...understands it has DMT in it's foliage so that's a big +.
Does Psychotria Viridis need artificial light and-or extra heating and-or humidifying to survive long, dark and cold winters?


Well...dark cold winters really doesn't give much information...so yes.

hehe, that would have been quaintly amusing to leave it at that...sorry, not tryin to fuck with you, just a little blazed. Anyway, to answer your question, it depends, but probably. You don't necessarily need artificial lighting, especially if you're going to leave your plant in the window, but it can't hurt. As a grower of indoor plants, I can attest to the benefits of artificial light. You're not growing in a natural environment and may as well accept it and give your plant as many advantages as possible. As to heating, it's the same thing. You should be fine unless you keep your house really cold, but to optimize growth you should get a heating pad designed for use with plants. Neither the light nor heating pad should be prohibitively expensive. Humidity shouldn't be an issue, as you can acclimate the viridis to your home's humidity levels or, if that's not satisfying, mist it semi-frequently to keep humidity up.

The short answer to your question is you can grow without additional lighting or heating...life is resilient and astonishing and you would not believe the things I have seen/heard plants survive or come back from. With that in mind, you are going to be cultivating a plant for your own personal use. Why not do everything in your power to give yourself the most out of your plant?
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MagikVenom
#18 Posted : 10/15/2009 10:02:53 PM

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neuro_rocket wrote:
MagikVenom wrote:


The grasses are all complete bunk in comparison to these two.




SWIM has to disagree. He is currently growing P. Brachystachys and D. Illinoensis. The grass has been growing for half as long and he has already collected enough material to try for an extraction and he has many new sprouts.

The Desmanthus takes a fairly long time to grow and is very hard to harvest without killing the whole plant.

Phalaris strains have been developed that yield a considerable amount of DMT and with proper stressing they can yield just as much, if not more than MHRB or Desmanthus, with much less expense than the first and much less effort than the latter.


neuro_rocket
I understand and if one is lucky enough to find a grass with a high level of nn dmt you are indeed correct. I have harvested from the wild and ordered a couple high alkaloid strains from the net I could never find a strain that was very potent and justified the elaborate extraction using many gallons of water. For me the huge voloume of the extraction was just to much work and to time consuming.

There may very well be a grass that is the holy grail of grasses that has a high nn dmt but I could not find it. But yes it may very well exist let us know if you have found it. Post any extraction results you get I am interested and many others as well.

Happy Growing to you Smile

PEACE
MV
 
SKA
#19 Posted : 10/16/2009 12:51:02 AM
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Allright from what SWIM read he thinks it is best to try and grow Psychotria viridis near a window, with artificial light to give it a little more hours of light per day in the short dark winter days.

Erowid says the DMT content of P.Viridis foliage varies between 0,1 and 1% DMT. Let's see how we can significantly increase that percentage.
This Erowid article explains that P.Viridis foliage harvested at Dawn and before Dusk were found to contain significantly more DMT than P.Viridis Foliage harvested during Midnight and from 10:00 am on during the hot parts of the day.

This suggests that DMT is formed in the plant when the Sun rises and when it sets. So perhaps, next to feeding your P.Viridis alot of Tryptophan, playing around with the lightlevels and times might be a way to push the DMT content higher.

Perhaps you could use artificial "dimmable" lights to fake dusk/before sunrise light levels for a prolonged time, so you give the P.viridis extra time to notice the dusk-like lightlevels and form DMT. If you kept it in this prolonged, fake-dusk/dawn and then harvest the foliage, perhaps it will have significantly raised the DMT content.

According to that Erowid article I linked to it should make quite a significant difference.
 
Infundibulum
#20 Posted : 10/18/2009 8:48:23 PM

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WTF guys, you know that amino acids like tryptophan are not absorbed from the roots, right?

So how are you going to feed the plant with tryptophan? Electroporation?

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