After reading through all AB teks available here, I decided to combine what I think are the best aspects of each. I wrote this for my own reference. Let me know if there is anything which can be improved.


1 Plant preperation:

- Obtain 50g mhrb (Mimosa Hostilis root bark)
- Cut root bark small and powder it to dust with a blender
- The plant material is prepared

2 Acidification:

Option A
- Have 200 ml of distilled water ready
- Add acid of your choice until pH 2-4 is reached
- Add the mhrb to the solution
- Shake hard to get it all mixed up
- Let it sit and soak for 3 days
- The acidified solution is prepared

Option B
- Pour 100 ml of distilled water in a a steel cooker
- bring to just boiling and stop the heat
- Add acid until reaching pH 2-4
- Add 50g mhrb and warm up for 2 hours in a heat bath
- Filter the contents
- Place the filtered mhrb back in the steel cooker
- Repeat process 2x more, 3 boils in total

- Discard the mhrb after the last boil
- Combine the solutions of all 3 boils
- Place them in an oven dish and insert it in an oven
- Reduce the volume to 200 ml at 70 deg C (lowest oven setting)
- The acidified solution is prepared

Option C
- Funnel the mhrb into a glass bottle
- Put 200ml distilled water in a pan and bring to just boiling
- Add acid until PH 2-4 is reached (60 ml of 5% vinegar will do)

- Pour the acidic solution into the mhrb bottle
- Put some tap water in a pan and bring to the boil, turn off the heat
- Shake the bottle and place it in the heat bath
- Shake/mix occasionally and re heat if necessary
- Leave to soak for at least 1 hour
- The acidified solution is prepared

3 Defatting (optional):

- This step is optional when using mhrb
- Place acidified solution (room temp) in a seperatory funnel
- Warm 50 ml of naptha in a heat bath
- Add the warm naptha into the seperatory funnel and close it off
- Gently roll making a figure of eight for 1 minute to mix
- Release the bottom valve every minute to let off built up vapor
- Wait up to several hours for a distinct separation to occur
- Help the separating process along by doing a heat bath
- Place the funnel into a sink running hot water for 10 minutes

- Funnel off the naptha and discard it
- Clean the funnel well
- Place the extraction back into the seperatory funnel
- Repeat this process 2x more
- The acidified solution is now defatted and ready

4 Salting (optional):

- Put 100ml of distilled water in a pan and heat to nearly boiling
- Weigh out 30g of NaCL salt
- Add the salt and stir until dissolved
- Pour the saturated saline solution into the dmt solution

5 Basification:

- Slowly add 50 grams of Sodium Hydroxide to 200 ml water (1g:1g NaOH to mhrb)
- Slowly add this to the solution
- Steer and keep on adding until pH 13 is reached
- At pH 13 the liquid will become gray in color and eventually turn black
- Add 140ml distilled water to the solution
- The volume should now be around 15ml:1g ratio solution to mhrb
- Cap the jar shut and tilt back and forth for 1 minute
- Stop and release the air buildup every couple of seconds
- The extraction will now look like a thick black tarlike liquid
- Warm the mix in the heat bath for 2 hours
- After 2 hours, cool to room temperature
- The basified solution is ready

6 Nonpolar solution:

- Warm 50ml naptha using a heat bath (1ml:1g Naptha to mhrb for 50g mhrb)
- For mhrb amounts of 100g or larger, use 0.5ml:1g
- Pour the basified solution in a seperatory funnel
- Add the warm naptha to the seperatory funnel and seal it
- Gently roll making a figure of eight for 30 seconds to mix
- Release the bottom valve every few seconds to let off built up vapor
- Place in a heat bath into a sink running hot water for 10 minutes
- Allow to settle for the seperation to form
- Repeat mix/settle 3x

- There should now be two layers visible
- Use the seperatory funnel to seperate the layers
- Get as much naphtha out without getting any of the extract
- Cutting the valve off a little later just to be safe helps
- Pour the top layer (naptha containing dmt) in a jar
- Pour the bottom layer (basified solution) back in the seperatory funnel
- Repeat the pulling process a total of 4-5x

- Make the last pull last longer than the other pulls
- Pour the naptha of all pulls together
- Allow the NP solvent to cool to room temp
- After cooling, store in fridge to aid in crystal forming
- Take out and evaporate down to 75ml using a fan
- The NP solution is prepared

Optional:
- Keep the last pull seperately to freeze/evap separately later on
- Check if the last pull has significant amounts compared to the others
- If its >0.15g compared to 1g from all other pulls combined, do another pull (or more)
- Pull until very little or next to nothing is yielded.

7 Naptha wash (optional):

- Add a pinch sodium carbonate to 50 ml of distilled water
- Use 25ml of sodium carbonated water per 300ml of naptha

- Pour the NP solution in a seperatory funnel
- Add the sodium carbonated water and shake visciously
- Separate the water from the naphtha and discard the water

- For the second and third washes use only distilled water
- Again use around 25 ml per 300 ml naptha
- Perform the same procedure as above
- Don't leave the water in the naphtha long, just in and out
- The NP solution is now washed and ready

8 Freeze-precipitation:

- Pour the NP solution into a pyrex baking dish with a lid and seal
- Place the sealed pyrex baking dish in a freezer (-20 deg C)
- Allow DMT crystals to precipitate in the freezer for 12-18 hrs

- Pour off the naptha, leaving the DMT crystals stick to the dish
- Place the dish with lid on back in the freezer upside down for a day
- Pour off the remaining naptha and take the dish out
- Use a weak fan to dry any remaining naphtha on the crystals

- Store the retreived naptha in an uncovered baking dish
- Place it in a well ventillated room for a week or so
- Watch it to see how much it evaporates
- Once its clouds again when you blow on it, freeze precip again
- Repeat until the yeild is very little
- Save remaining solvent, reuse for later extractions
- The DMT crystals are prepared

9 Crystal wash (optiona):

- Dissolve crystals in minimal amount of hot naptha
- Use 20ml naptha per 1g DMT crystals
- Add a small quantity of crushed activate charcoal to the solution
- Warm in a heat bath for about 10 minutes
- Swirl and watch undissolved sludge form at the bottom
- Remove charcoal and sludge by decanting
- Allow solution to cool and reach room temperature
- Transfer to the fridge for a couple of hours
- Transfer to the freezer
- Add crystals to help seed crystallization
- Wait for crystals to precipitate out of the solution
- Collect by filtration as before
- If unsatisfied, reduce naptha volume and repeat
- The DMT crystals are now washed and ready

Update: the above steps have been modified to the answered questions below.

Acidification:

How much water is best to add in proportion to the MHRB to acidify? Is it better to add as little as possible, just covering the bark, to end up with less volume later on? Or will the water be saturated too fast if the volume is low, resulting in less dmt entering the acidic water? Is 1mg:2ml MHRB/acidified water a good ratio?

Could simmering the water for a long time risk destroying yield? What is the best /safest /easiest way to reduce volume: simmering in a steel cooker, heated in a (crock-pot) slow-cooker without lid, or in the oven at around 80-90 deg C?

Basification:

What is a good proportion to create the lye/water solution, to pour into the acidified solution? Or beter yet, can you add the lye powder straight to the acidic dmt solution, using a digital pH meter to measure pH? Keeping the water volume down.

Nonpolar solution:

Adding too little naptha to the basified solution results in less dmt being pulled in the naphta. Adding too much naptha to the basified solution results in less dmt being precipitated after freezing. Best is to add a bit too much, then evaporate down. But what is in theory the perfect proportion of naphta to mhrb without needing to evaporate, providing mhrb contains correct amount of actives? 3x 100ml naptha pulls for 100mg mhrb? Does this sound reasonable? Is increasing it to 3x 150ml pulls efficient or too much naptha? Is evaporating it down from 450 ml (3x150) down to 150 ml (1/3 original volume) sufficient?

Sources:

https://www.dmt-nexus.me...aspx?g=posts&t=36239

If your committed to doing an a/b don't use a coffe filter to get the spice out of the naphtha. There will be remaining naphtha in the spice & it will melt into the filter very fast.

Leave it stuck to the jar & pour off the naphtha. Then stick the jar w/ lid back in the freezer upside down for a day, pull it out & use a weak fan to dry any remaining naphtha on the crystals for a while.

Don't use a mason jar for freeze precip, its hard to scrape. Substitute the mason jar with a pyrex baking dish with a lid.

Other than that it looks good.
Water ratio should be 15ml:1g mhrb
Lye ratio should stay around 1g:1g lye to mhrb
Solvent should be 1ml:1g mhrb, less can be used 0.6ml:1g mhrb

You will very likely have to evap some naphtha to retrieve all your spice, after freeze precip just leave the baking dish uncovered in a well ventillated room for a week or so, watch it to see how much it evaps.
Once its clouds again when you blow on it you can freeze precip again, repeat until the yeild is very little, save solvent, reuse for later extractions.

If this is your first extraction do not use 200g mhrb. Try 50g. Less loss if you screw up.
When you get your extraction working nicely you can scale up.
Cybs tek makes it much more simple & yeilds high.

Thanks for your answers concombres!

I inserted your corrections and checked out Cybs tek. Probably going for Cybs ion tek. With additional freezes after as you mentioned.

I guess you could use your blowing on it to see if it clouds tip to tell how much you should evaporate prior to freezing the first time as well, right?

"Water ratio should be 15ml:1g mhrb". <- Is that in total or per pull? Why 15ml?

If per pull. that's using 3 x 1500 ml = 4.5 liters of water per 100g mhrb. Even doing only 1 pull is 1.5 liter. Reducing consumes time and energy. Other teks state smaller ratio's such as 2ml:1g.

Is there a proven yield increase doing 3x heated pulls? Cybs tek says to do 1x heated pull for an hour with around 2ml:1g acid solution to mhrb ratio. (Cybs max ion tek says heat for 8 hrs).

When basing, is there a disadvantage about adding the lye straight to the acidified dmt solution? Provided you have a pH meter and stop at pH 13?

Solvent should be 1ml:1g mhrb. <- Thats per pull, correct?

Thanks again!

Yes you can use blowing on the solvent to some degree but it is not 100% perfect. You want a small volume of solvent before the freeze. I like to combine all 3 pulls & then evap down to 75ml, freeze, then evap down to 50ml & refreeze.


The 15ml per gram of mhrb is the total volume of water for the entire extraction. Your boils if you do them should be combined & reduced down until they are 15ml:1g but with cybs tek boils are not needed.
The max ion tek is not reccommended fpr beginners, i'd say use the hybrid atb, it's provided me with 2% yeilds every single time but then again everyones bark is high quality so you may get less.

As for the acid soaks, i always do 2hrs in a heat bath. Thats before the basic solution is mixed so it's less volume, but after basification you'll have 750ml total per 50g bark, which is 15ml:1g.

Heated solvent pulls do seem to increase yeilds per pull but if you'd like you could just do more pulls without heat for the same yeild.
Too much heat pulls yellower spice.

do not add the base directly to the acid pull, it throws off the water ratio if you don't add more & the lye creates alot of heat when it reacts with water & can break the jar, mix it in the ratio mentioned in the tek with water (lye to water never water to lye) in a pyrex measuring cup & stir until it all dissolves & the water clears.
Then mix it in the acid soak, make sure it doesnt get too hot & explode.

& yes your solvent ratio is right.

Greatly appreciate your help! That pretty much answered all my A/B extraction questions!

Next step is checking out FASA, full spectrum extract from D-lemonene, and endlessness eco-friendly 'ethanol, vinegar and sodium carbonate' tek.

But my first tek will be 'Cybs Hybrid ATB Salt Tek' with 50g mhrb. Combined with: fridge decanting, defatting, post freeze evaporation and re-freezing, and crystal wash. As written above. When successful, I will re-do with 100g.

I'd say do cybs tek a handful of times & get comfortable befor moving to 100g.
The food safe teks are nice, but can be a little more complicated and/or take more work to yeild high but are well worth it once your comfortable extracting.

You seem to be taking in alot of information & making things seem more complicated than they are. I did the same thing when i first came here.

Once you get the hang of things its alot simpler. If you'd like to learn a/b extractions i'd reccommend doing a few extractions on syrian rue.
It's dirt cheap & helps you run thru the process a couple times & become comfortable while deveoping a technique & figuring out what works for the equipment you have even if it's just pots & jars.

If you manage a successful extraction from rue you can even feel out the dosage of your extract & mix in some spice for pharmahuasca sessions

There is alot that can be done extraction wise with all the different teks & different routes of administration dmt offers.

You nailed it! I do tend to overcomplicate haha. Better to just start and learn as you go along I guess.. I will do that. And great idea to experiment on rue!

One thing still confusing me is that cyb says to use the same amounts for 100g as for 50g. In which case you would get a 7.5ml:1g based solution ratio instead of 15ml:1g.

But yeah, I should stop the thinking and just do some 50g extractions first

Thanks again concombres!

It's the same ratio but not the same amount man.
You take the ratio & multiply. If the ratio is 1g lye : 1g bark (50g lye for 50g bark) then for 100g it would be 1g lye x 100g bark = 100g lye.
15ml water per g bark = 15x 100ml = 1,500ml
1ml solvent x 100g bark = 100ml solvent per pull

The base solution may throw you off a bit because its mixed in with the acidic solution.
The total volume of water (ex. The acid bath + the basic solution) is the ratio of water to bark.

& it is good to understand the science behind all this, but i find it was very confusing initially & in order to understand it better i had to not only research but learn hands on as well.

You may mess up a few times & it may cost you some money, but it's a skill like anything else & if you keep at it you will eventually get it right & be rewarded for your effort.

Understood, just that cyb confused me a bit heheh. But now I get it..

I will my post extraction results, hopefully within a month..