We're still discussing it over there, apart from a couple of people this hasn't worked, think we need a few people off here with the expertise and know how in chemistry to take a look

if a compound has a charge state other than 0 on any of its side groups across the pH range, you will have issues
isolating it from other compounds using simple extraction techniques.

Benzyme you exactly the person who can sort this out once and for all, that's if you wouldn't mind looking at the thread I posted, I just dont belive the op and the extraction he did which is on page 9 of the thread to get crystals

Primal soup from the shroomery.. Yes no mushrooms eaten.. i find human bufo very interesting !
but 34grams per liter seems like way to much tobe true.. Bufotenine active dose 5-100mg.~Ott


Here is the research papers being referenced...

http://www.fsijournal.or...%2800%2900213-9/abstract

Quote:
Abstract

Active compounds of some mushrooms e.g. Psilocybe cubensis, Paneolus subalteatus or Stropharia coronilla, the psychotropic agents psilocybin and psilocin, have hallucinogenic effects. In one case of ‘magic mushroom’ intake, we had to analyse blood and urine. Psilocin was detected in the urine with REMEDi HS. Most of the psilocin was excreted as the glucuronide. Therefore an enzymatic hydrolysis should be the first step in analysis. Free psilocin was determined at a concentration of 0.23 mg/l while the total amount was 1.76 mg/l urine. The concentration of psilocin in serum was too low for detection with REMEDi HS. We proved a GC–MS-method with d3-morphine as internal standard and silylation with MSTFA. Similarly to urine, most of the psilocin in serum was found in the conjugated form. The concentration of free psilocin was 0.018 mg/l, that of total psilocin, 0.052 mg/l serum.



http://informahealthcare....1080/003655101753218319

Quote:
Research Article
Determination of potentially hallucinogenic N-dimethylated indoleamines in human urine by HPLC/ESI-MS-MS

2001, Vol. 61, No. 7 , Pages 547-556 (doi:10.1080/003655101753218319)

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T. Forsström, J. Tuominen, J. Kärkkäinen


A new method for the determination of N,N-dimethyl-5-hydroxytryptamine (bufotenine), N,N-dimethyltryptamine (DMT)*, 5-methoxy-N,N-dimethyltryptamine (5-MeODMT), and N-methyltryptamine (NMT) was developed using highperformance liquid chromatography-mass spectrometry (HPLC-MS). Identification of the analytes is based on liquid chromatographic retention times of analytes and two fragment ions produced by a triple quadrupole mass spectrometer. Quantification is based on electrospray ionization (ESI), and multiple reaction monitoring (MRM) was also utilized for getting better selectivity. The analytes and internal standard were separated from the urine matrix by solid-phase extraction (SPE). The method was applied for the determination of these compounds in urine samples of patients from surgical, medical and psychiatric wards. Of the dimethylated amines, only bufotenine was found in significant amounts (up to 34 w g/L). In keeping with our earlier results, the bufotenine excretion of psychiatric patients was found to be higher than that of the somatic patients. Method, procedure, considerations, statistical evaluations and urine sample spectra are presented.

I don't believe it either. you wouldn't even get psilocin crystals like that it f you did the same procedure on mushrooms.

Benzyme what about bufo , 34 g per liter , thts insane

if this is true this is going to be .......

it's probably ng... nanograms/L.

what that guy likely has in the jar is urea hydrochloride

Whats with the thumbs down man? Sounds like good news to me.


If that's 34 g/l, it means you can trip balls/end up in the hospital/die for real by drinking 1 litre of your own pee. So it's probably not true.

Some good comments regarding this matter now, at least we'll be able to put this matter to bed soon

Seems like that guy received a ton of hero worship without any evidence and a three sentence tek with tiny pictures. The MOD slipped out the door with just one try and no results. This is why I love the Nexus -- We require evidence to support far-out claims and do our best to reserve judgment until claims are tested.

Not trying to bash the other forum... But to me these are key components to furthering the cause. Extraordinary claims require extraordinary evidence. 19+ pages is way too much noise to signal for something like that. (The little GIFS after every post drives me bonkers as well.)

Nice job trying to bring science into the discussion.

I totally agree alpha and 25 pages later he's still trying to justify his claims, if you need answers bring it to the nexus and i'm glad I did, unless someone has actual evidence then this has been put to bed by the nexus community

Of course ngs lol i didn't think of that, it could even be mgs.

i don't know about Pks, just curious would the urea stay in the water with a non polar solvent pull?

mg/L sounds kinda high. people would be walking around in a perpetual daze.

and urea is highly water soluble, of course, especially soluble in aqueous hcl.
something in his extraction doesn't add up. he assumed psilocin passes through the liver and kidneys 100% unchanged.