I was wondering if there is any complication with starting a shrooms cultivation with a gtg from an already colonized pre made substrate (classic grow kit). This is because i have no spore print\syringe to start with but i want to speed things up... Just transfering some spoon of the premade substrate in a few sterilized grain jars and then go on with them.

Grain to grain transfer, just as i explained before.. check this out!


http://www.shroomery.org...wflat.php/Number/1348626

No problem doing a grain to grain transfer with a purchased grain "kit". Just make sure you're transfer happens in a glove box, under a flow hood or some other sterile environment. Follow sterile procedures and you should be just fine. I guess you're going from a "sterilized spawn bag" to jars?

Yes i am.
I am not going to do it in an isolation chamber, since i have not build it yet, but i work in a really clean enviroment.

by the way, what would be the ideal moment to transfer?
My grow kit arrived about a week ago and its very colonized (i knew the right moment would be when the container is 100% fully colonized but i have never seen a pre made grow kit fully colonized (totally white), it seems like they fruit before to reach the fully mycellium colonization...(and not like the classic fully white aspect of the homemade PF jars).

Can i transfer it even if its only 75% ? in this case what would be a good transf-ratio? a full tablespoon for a 250 ml jar sounds ok?? or could i use less?

Using a glovebox will save you alot of trouble & so will not cutting corners.

I've worked with grow kits & they suck compared to preparing your own grain jars. I'd advise no matter how clean your enviornment is to use a glovebox or yoi WILL get contamination.
You have no idea how many mold spores & bacteria are flying around a room at any given time & its only a matter of time before some fall on your substrate & take hold, especially substrate from a grow kit that will never even reach full colonization.

You can't g2g from a growkit because it is not sterile. When you g2g you have to be very sterile and do everything in a still air box otherwise you will end up with contaminated spawn.
Fully colonized spawn is very resistant to mold whereas clean, sterile and uncolonized spawn doesn't have any resistance towards molds and other organisms.
When a growkit is made, it is subjected to air and all kinds of spores. That is not something you want to mix with sterile grains.

I won't elaborate on what others have said, their concerns are legit. However, making a glovebox can be as easy as using a clear plastic trashbag or a cardboard box with once side cut out and covered with plastic wrap or two holes cut out for hands and one side covered in wrap. It will make a big difference.

I would NOT do the transfer until I mixed the grain up (while still in bag) after its been fully colonized. Usually you wait about a week after everything turns white then shake it up. When you use jars you can bang them against a bike tire. Once its broken up I'd give it a few days to recover but not become one solid mass again, then do your transfer.

If you transfer before 100% colonization you WILL have problems and you'll lose your whole batch. I know it can be a pain to wait but its worth it. In the meantime you could look at some gloveboxes and how easy they are to make, just a cardboard box is all.

good luck, what strain are you growning? have you done the pftek yet? are you planning on going to manure?

yeah, about that will be fine, if your grain is small like wild bird seed, even if its rye berries it
should be enough.

If you've purchased a growkit that you only have to dunk and fruit, then you cannot g2g. If you have one of the kits where you get a bag of sterilized grain which you have to inoculate yourself, then you can g2g.

If I understand your posts right, then you have the type you only have to dunk and fruit. And they are no good for g2g.

Take some prints from the fruits and start a PF tek grow from scratch. It's easy and cheap.

truth

i need to read better