pans are strong as it is, adding tryotophan likely does nothing.

why?

because the fungus derives most of its own tryptophan. decarboxylation of tryptophan is a rate-limiting step, so adding more tryptophan doesn nothing to move the reaction forward. add tryptamine or NMT would increase psilocin production, but it needs to be done at low concentration, like 180 mg/100mL water, or you run the risk of stunting growth.

tonyx3
Water your substrate with it. Some like to mix it in. Recent grow logs I've read trying this added Desmanthus illinoensis or mimosa to the substrate, not tryptophan. Probably because of what benzyme wrote. Watch for his advice on concentration. Part of my outdoor patch dwarfed after the first flush.

Nothing to be sorry about, It's not personal.

I should have differentiated what is toxic to the mushroom is not toxic to us.

Organic waste is non-toxic to a mushroom and can be metabolized.
Human-made toxins produced by complicated human processes like industry are inorganic and would likely not be metabolized.

I do realize that they metabolize organic waste into simple chemicals that produce more complicated ones. Most likely as defense or for symbiosis, but that is beside the point and irrelevant.

I'd like to see them do that with any man-made toxin that is far removed from anything resembling organic waste!

So I think they can do both.

This article touches upon both processes at work:



Here is the work Stamets did and wrote:


Sorry I didn't have his writeup earlier. NOw we all can Haz.

I'm sorry if you have not enjoyed my haphazard thought processes. I will work on making them more precise and concise. Peace.



~Ø~

So we agree I think?



Going with Stamets on both.

Dude knows the score.

What are you saying? theres no problem
The heavy metals are removed with the intact mushroom fruits
If the shrooms dont metabolize the junk they are taken away from the site. Either/or.
One can pick and choose depending on needs.

Dude, that's what I said. Heavy metals and radiation are a exceptions.

Gotcha dude.

Would you suggest or were you suggesting its cuz of their small size allowing them to filter and bypass the halting of the metabolic process?

I get now that I was missing what you were suggesting, that they do one or the other but not both generally and that heavy metals are the exception. Radiation is much easier to understand in terms of that.

But I also feel there is something to the fact that heavy metals really can't be broken down much further in this process cuz they are elements.

The reason I like this particular puzzle is because I like the idea that people who have worked with these substances are most likely emotionally/spiritually more mature folks who wouldn't experience much pleasure from telling lies, and that makes me want to try to understand that maybe there is a way this is a possibility and some things are overlooked.

Its strange to watch people try to lie for whatever reason when they are tripping hard.

like somehow "we attempt to create mushrooms fed ayahuasca and you better believe me when I say they are dank bro!"

As if there was motivation by trippers to do that? It seems it was not money unless they wanted to attract tourists to eat their mushrooms or something, I would hope it wasn't egoistic because those same cool mushrooms can shatter that ego they are tryna build. And thats funny lol.

I have to also hope it is not psychosomatic

But regardless, I agree with Koqan:



I am not a good enough biochemist to know why ben would say "nothing about mushrooms absorbing harmala alkaloids, but metabolyzing them isn't likely. enzymes are largely substrate-specific." Its clear to me that they are substrate-specific, but also that a slow introduction of different organic matter can alter their abilities to consume organic matter.

It makes sense that introducing tryptamine could trigger them to produce less on their own but I feel like they couldnt evolve quickly enough to make those changes in endogenous levels. It also kinda seems like they would break down tryptamines to tryptophan and then back to usable stuff. They could break down those chemicals all the way to simple sugars and then back to whatever, I am guessing. I don't know a lot about that process.

Like overall do they generally go to sugars and then make stuff from "scratch?"

I can't tell also if there is a point where they go into a kind of survival mode- perhaps they override the filtration of chemicals when they "hit a wall" and can't further colonize. Like a last-ditch effort to spread spores to the next spot where food can be produced and absorbed. Like where water is needed rapidly and absorbed readily in the last few days, during fructification et la chute des spores.

There's a lot I want to look into to understand: do mushrooms metabolize to the most usable chemicals, or down to basic building blocks of organic matter? It seems tough to know the details of that process without extensive chemical analysis.

It is known that introducing a new organic matter slowly can lead to successful alteration of their abilities to metabolize new organic matter.

Damn I just lost the end of this, I'll try to reconstruct it.

So which one are they doing?

If its metabolization, the experiment wouldn't change anything much I'd suppose, as the encoding would permit them to digest usable stuff into other usable stuff, and Nexians seem to agree that filtration doesn't permit chemical compounds to be drawn into the substrate directly. If they do a survival-modey move to override the filtration and permit more water to be used, like sending out the men in lifeboats cuz the ship is a-sinkin'.

The fungus would have to be harvested and tested at various stages of growth for the whole chemical analysis.

The questions/variables are like: Is DMT or harmaline absorbed into the substrate at any level of growth as anything other than simple organic materials like sugars? I guess this is where the test for levels of more psilocybe or harmaline in the fungus comes in. Assuming no, are the chemicals absorbed anyway at the later stages of fruiting?

And, if they do metabolize the new isolated chemicals, into what? Like what chemicals are detected in one vs. control throughout the stages of growth?

This is a really interestin' science project. A lot can be going on in this gray area of sorts.

So I'm all like "WHAT DUDE? WHAT IS GOING ON AND WHY DOESN'T SOMEONE KNOW IT YET?"

Something can be gained from this even if the girl was an outright insecure lying greedy hippy who worked with Shazam in other Americas.

How far does the breakdown of chems in the metabolic process go, and if they are substrate-specific, what are the substrates utilized as, and is there an override point where other stuffs can sneak in?

The variability of toxic mushrooms in nature even with one type of mushroom seems to suggest that various levels of stuff could be going on.

I get that my style of questioning might not be the most direct upheld by the academic world, but learning is in asking the right questions. I'm not as into the whole "who has built a vast wealth of knowledge enough to rely on old facts to understand this?" As it would rule out the need for experimentation. So if the whole "Dude dont you get what I'm saying here..." stuff was left out I'd be fine with it. Either way its all fun. Sorry to be frustrating. Developing new theories relies on being wrong more often until a correct interpretation is attained.

~Ø~