THis is where the topic changed, and the answer is most likely: none.

Mechanical wicking action leading to residual substances trapped in the fruiting bodies was the only action, basic hydration.

The whole DMT supplementation part has kinda been covered before on here which I am guessing is why so many doubted these claims after this question about metabolic pathways was asked, but it was never suggested by the OP.

Double-Huasca.

Bassnectar- Dubuasca

Is there any evidence that this actually happens? That the mushrooms "wick" up moisture along with the compounds contained therein, and simply store said compounds within their fungal tissues (without metabolizing, excreting, or otherwise affecting the compounds in question)?

I'd like to see a source for that claim

yeah, it's pretty well known.

Like I had said, mushrooms are at the forefront of viable options for the removal of toxins from the environment.

They are basically hydration wicks while they grow.

I'm getting booted off this hotspot due to time limits but I'll get back to you on it.

Would the Staments New Orleans effort to remove toxins from the soil be a good precedent for this being a possibility?

My recollection is that mycoremediation doesn't result in the toxins being stored in the mushroom, but results in the metabolization of at least some of the toxins. This is why I phrased my question above as I did. I don't know of evidence showing mushrooms to simply suck up and hold onto things from their environment, but I'm no expert on this.

Certain waste material might be absorbed by mushrooms but only when it serves the fungus to do so, such as breaking things down into usable fungal sugars. This does not mean they will just store anything so far as I understand it.

My understanding (just by looking at the detectable levels of mushroom metabolites) is that 1) either no DMT is produced in the mushrooms during psilocin/psilocybin synthesis and/or
2) if psilocin/psilocybin involves DMT, then that is a more unfavourable pathway.

My reasoning is that since we find baeocystin and norbaeocystin in the mushrooms, a likely biosynthetic pathway involves tryptamine 4-hydroxylation, followed by optional phosphorylation, and topped with methylation and di-methylation of the amine.

But why isn't it likely? cytochrome P450 enzymes are abundant and present in the fungal kingdom and the CYPs are notorious for being particularly flexible with their substrates. We metabolise harmalas, so why not mushrooms?

E.g. CYP2D6 that metabolises harmalas in mammals has fungal homologs...

Is there any evidence for that? I remember reading something like that in Stamet's book, but Stamets is notorious for stretching his pro-mushroom arguments and sometimes uses dubious lines of evidence (e.g. not anything from his mycelium running book has been peer-reviewed and/or published).

During growth water is absorbed by mushrooms regardless of what else it contains. if it doesnt "kill" it it is retained in the fruiting bodies. if it dies then initially it is still absorbed. This is well known and you can look up "mushrooms as a means of contaminated soil cleansing." Once again nobody said the mushrooms always survive this contamination, it is irrelevant as the fruiting bodies whither so quickly after fruiting and It is VERY unlikely that they metabolize most toxins as the man-made chemicals are too complex to be usable. We should remember that mushrooms have been a vital part of a natural, organic environment since nearly the beginning of the Earth. In soil cleansing more fungus can be introduced, can it not to perpetuate the chemical removal?





But why isn't it likely?

They need water to survive, water can be contaminated and if "tainted" water is absorbed the mushrooms either:

A)metabolize it if it is useful, which more complex chemicals likely are NOT because the mushroom hasn't had the exposure to evolve to include that kind of process of utilizing man-made waste, it was never their role in nature.
B)absorbed by necessity because the mushroom needs the water to reproduce and perpetuate itself
C)selectively don't absorb it by some means and die? As if with absorbing the water, the mushroom "knows" which water is good for it and halts the process?

Evolution and also reason dictates B. I think it is that simple.

As to why they don't break down harmalines to sugar and make them into tryptamines, I guess that question stands, but we all agreed from the beginning that that part is not likely, so if you can agree with B then the rest is succinct.

UPDATE:

Three of us ended up taking the special aya mushrooms last night. We only did 1 gram each and it hit us HARD. It was one of the most spiritual experiences of my entire life. There were about 5 other friends in the group that were sitting around the camp fire doing a mild acid trip. My trip was so intense with energy... It was as if I(?) could feel everything around me.

So many things happened so I'll only tell you about the most intense. During the peak, I felt some negative energy coming from the 5 people sitting by the campfire. Normally I would walk away but this time I felt like I was being pushed over there to confront it. I picked up a large stick and grabbed it firmly, thrusting it into the earth with one hand. With my other hand, I reached towards the 5 people and sucked all of the negative energy out of them. I allowed it to flow through my body and mix with the positive energy that I was extracting from the ground.

I then looked up at the milky way galaxy and let out a HUGE deep roar of ecstasy for about 2 full minutes while tears were running down my face. I was releasing their cleansed energy through my mouth to be recycled into the universe.

When I started my roar, they began to giggle a little. But the louder I got, the further out into space I was taking them and the more positive and uplifted they got. That part felt like a non-visual DMT breakthrough with passengers on my back. Almost like I was on my way out of my body but not completely. But it was very difficult... I felt like if I wasn't absorbing positive energy through that stick, I would have died. Later they told me they weren't sure if I was having an orgasm, dying, or being possessed. One of the more experienced trippers said "nope, he was just very much alive".


Needless to say, they worked VERY good. I am going to try to find out more about them.

Here's some pics. Maybe someone can identify them.

This is really exciting! Although this is one specific experience there is a lot to learn from how you describe the dynamics!
You were able to enter into a mindset where you were healing others, also where you were working intuitively and passionately with your voice!

This can happen with mushrooms on their own but my response at least is that there does sound like some overlap with the ayahuasca being involved.
Bearing in mind The Cosmic Serpent book is my main reference for understanding ayahuasca, during the mushroom growth, wouldn't some of the many plant DNA types within the brew mixture enter still living into the mushrooms and quite possibly interact with the mushroom DNA? So far no one is sure how strongly DNA experiences and perceives reality, but, butterflies are able to mimic eachother and orchid flowers are able to resemble bees. The Cosmic Serpent emphasizes to some extent that the concept of DNA being as fundamental to the experience of ayahuasca as the tryptamine and harmaline content seems valid according to different accounts from ayahuasceros.

Sending love, hoping you will be able to keep exploring with this unique preparation and care being given to these mushroom teachers!

On MDA and ketamine once a "column of knowledge" descended into the room with rainbow spectral double-helix translucent appearance and revealed itself to be very pertinent and relevant to the chemical-spiritual communication, admittedly and I was astounded by its beauty and intricacy...


If you watch the movie "I AM GAIAM" it is an excellent documentary about how cooperation is the rule in nature via communication, and not dominance. The majority disregard the self-centeredness of any alpha male tendencies. How birds and schools of fish veer in another direction in an instant, and how herds decide when to go to the watering hole together. It is absolutely excellent. They bring it full-circle and state that humans must learn to do that or perish. I agree.

Theoretically it makes sense for DNA and -amines to be able to interact as they are the basic parts of organic material.



I think we are looking beyond the fact that these DNA can interact on the physical plane to access the deeper essences, and they don't need direct communication, or are in the way of DNA interacting directly.

oooh I forgot... studies have shown dna can modify through our consciousness through such input as a single spoken word.
link to this later, i am at work at the moment but all excited. good stuff, with the amines and the protein building blocks. Frontiers of knowledge and all.

Although DNA may be interacting, I think plant consciousness and human consciouaness are interacting symbiotically, and DNA records the changes or results like a record of the alteration.

Sorry but this is such a gross chunk in misinformation.

You know metabolize means to break something down into something usable as food, right? They don't just wick up and use toxins as building blocks, they break things down into food.

Ok, I tried that search, didn't see any specific study under that name. Why don't you link us up ? I can't find anything that backs this up. Radiation and heavy metals are the problem, anything else is either food or not food. It will colonize or it won't. If it cannot be used by the mycelium, it won't be.

Mushrooms grow on cow cow sh*t. They grow on rotten unsterilised substrates surrounded by thousands of bacteria and competing toxic fungi producing all manner of harmful substances.

Mycelium does not just just drink up toxins, it either metabolises it, fights it off, or nothing.

People would just die constantly from non toxic species if what you are saying was true. Your average supermarket mushrooms would be so rich in bacteria that they would guarantee you get food poisoning every time.

Gonna quote ol' trusty infallible RR here, too:

this sounds like one of those "hey, what if i tell this guy this is what happens and i can charge him a buttload for it?"
watch vices new documentary "whats in my baggie" people do it all the time, next time you get molly it could be bath salts. everyone will lie and play with the truth just to get more money

i hear what you're saying but in this case, money wasnt involved

hehehe, cant go wrong with anything free!

Growing with harmalas alone could be a nice experiment to make things clearer.
L-tryptophan is known to increase psilocybin production.
I've grown pan cyans outdoors watered with mimosa juice and it was very powerful. I invited 2 friends to take them with me. One of my friends had taken ayahuasca a day before and expected nothing from the mushrooms. They called two more friends. I thought we barely had enough mushrooms for 3, now we were 5. Now I expected nothing of it. I also ground 40g mimosa trunk bark in cold water once and filtered. We split this in 5 equal doses and drank. But as far as I know, 8g mimosa trunk bark without a MAOI is not enough to be psychedelic.
We went deep into infinity that day. Everyone had their minds blown. The guy who had taken ayahuasca said it had been the deepest trip he ever had.

ha, I didn't mean oxidation. sorry, I should have specified that
I speculate that such enzymes are responsible for giving psilocin extractions their greenish tint.


what I meant was enzyme using harmalas as a substrate for hydroxylation at the 4-position (relative to the pyrrole nitrogen), it's not likely with a beta-carboline.

How does l-tryptophan increase psilocybin production? Do you mix it into your substrate or something?