Testing with a single reagent provides very limited discriminatory power, and single measurements leave no room for error since there's no additional checks done to ensure that the result is valid.

Contrary to popular belief these test reagents do not positively identify any drug. It's not a case of test with reagent X and if it turns a certain color then it must be substance Y. The testing is actually reductive. What we're trying to do is eliminate other possibilities with contradictory results up to a point where it seems most probable that one drug is present.

First we have to identify the other substances that might be present and that we need to be able to distinguish. In the case of LSD we're trying to distinguish it from other high potency hallucinogens such as the DOx series of psychedelic amphetamines, benzodifurans like bromo-dragonFLY, the NBOMe-2C series, 5-MeO-AMT etc.

Ehrlich reagent is an indole-reactive test. Ehrlich would therefore be expected to give a positive response to both LSD and 5-MeO-AMT since they are indoles, but not to the DOx series, bromo-dragonFLY or the NBOMe-2C series. A positive test with Ehrlich reagent would therefore make the presence of the latter substances improbable, while not contradicting that the substance may truly contain LSD.

We then need a second test to distinguish between LSD and 5-MeO-AMT. In the presence of LSD Marquis reagent turns olive green to black. Depending on the concentration 5-MeO-AMT should give a golden/brownish hue instead. It's important that the test is done at a suitable concentration so that the end color is not too intense, as deep gold/brown may look almost black. Again, an olive colored result would make the presence of 5-MeO-AMT improbable while not contradicting what would be expected if LSD were present.

Mecke reagent could then be used as an additional check to increase the confidence of the results. Mecke would be expected to turn olive to black with LSD. Any other color change would suggest the presence of something other than LSD.

So if after three tests we haven't had a contradictory result then it seems probable (though still far from certain) that LSD was the substance in question. One should still take care when consuming a new substance though. The test results are not 100% reliable, especially if the substance was a mixture.

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LSD fluoresces (glows) blue to blue-ish white under longwave (360 nm) UV light. Obviously other things glow under blacklight as well, so just the fact that it glows doesn't mean it's LSD, but if it doesn't glow, LSD can generally be ruled out. The glow should be apparent in both crystal form and liquid form.

Whether or not this test can be used for LSD liquid that has been dropped onto other materials such as blotter or sugar cubes is more complicated. There are reports of being able to hold a blacklight close to a hit of blotter in a dark room and see a glow in the spot on the paper where the acid was dropped. However other people report that the concentration can be low enough that it doesn't appear. An additional complication is that some paper or inks themselves glow under blacklight, so a fully glowing hit of blotter (rather than a glowing spot on the hit) doesn't identify the presence of LSD.

Nowadays, 95% of what is called LSD, isn't. - Timothy Leary.

1) gas chromatography–mass spectrometry (GC–MS)


2) HPLC + UV-vis spectroscopy

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