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Ive detected Capitate-Sessile Trichome Glands on the underside of Reed Canary Grass leaves Options
 
Xare
#1 Posted : 7/15/2012 7:08:49 PM

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Hello forum,

I come here seeking a bit of wisdom from you DMT Sages.

Ive had a fascination with DMT for awhile now and I believe it to be the Philosophers Stone and the True Eucharist.

My curiosity lead me out into the woods this morning in search for some local flora that contains DMT.

I knew that nearby was some tall grass I suspected was reed canary grass. I went out to harvest some and to use a specimen for positive identification. I saw three kinds of grasses in the area and I brought back a sample of each one that looked like a tall type of grass. After bringing them all back and comparing them with photos on the internet I could quickly discount two of them and the third I found to be Phalaris Arundinacea.



Now that I had found my prima materia and a place where plenty of the plant grows I wanted to start to experiment with the plant to make something from it.


So I was reading around and thinking when the idea struck me to use my handheld microscope to inspect the plant.

See iam a Cannabis guy... and we sometimes use microscopes to look at the glands on our plants too see how mature they are for harvest.

What I have is a digital microscope that I can take pics with and video. Ive used it before for looking dry sift keef. Or some Hash, sometimes I make water hash. Ive also used the scope to look at the glands on Catnip. And ive made catnip hash I guess you could call it. Basically its an extract from the catnip plant, a concentrate of the essence of the plant that is made up of a plant terpenoid called nepetalactone. Which was first discovered through steam distillation.

That was some of my alchemy experiments, as I have no formal training in chemistry. Ive not done too much other then make some Oils from cannabis and turned that into Spagyric which I used to remove freckles on my arms, but that is another story Smile



So I guess I have to explain a bit about water hash for you guys to understand what iam going on about here...

Well when your making hash your trying to make an Extraction of the cannabis plant. To gain its essence. Which basically is a Concentration of the Trichomes of the plant. Which are usually dissolved into a solvent and then the solvent is collected and evaporated.

But your not using a Solvent to make water hash.

Actually your using Water which is a solvent, and Temperature, agitation, separation, and Gravity. But the gland is not water soluble.

Your applying some simple alchemical techniques to extract the essence of the plant. Its best to freeze the material. Because the trichome gland heads have a thin waxy coating that surrounds all of the alkaloids. It becomes firmer at the lower temperature, and the stem is more brittle so it aids in the glad detaching from the plant matter with agitation. We use ice in the water to keep the mixture cold during the extraction. The ice can also aid in the agitation to break away the glands from the plant through physical rubbing. This gets mixed for about 15 mins with a power mixer to break off all the glands.

Then the glands get released in the water and through gravity they sink while the plant matter floats.

We use bags with incrementally smaller screens in them to filter away the plant matter and ice leaving behind the glands to be collected in another bag.

The ones I used are called bubble bags. But there are other types of Herbal Essence extraction kits made by other companies as well.

I use this to make all the Hashish that I Vaporize.

And I propose that with the discovery of the Trichome Glands on the underside of Phalaris Arundinacea that I can make a very potent type of Extract.


The question I put to you is if you think these living drops are safe to ingest ?


What you are looking at here is a 100x magnification of the tip of the underside of a reed canary grass leaf. And you see in all the channels all along the leaf are filled with Glands that contain DMT.



Ive put together a little slideshow of the macro shots I just took with my microscope of the reed canary grass.

Some pics are 200x magnification which means the glands are just over 25 microns.

http://img24.imageshack..../9029/13423711462m3.smil
 

STS is a community for people interested in growing, preserving and researching botanical species, particularly those with remarkable therapeutic and/or psychoactive properties.
 
The Day Tripper
#2 Posted : 7/15/2012 7:21:37 PM

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I see 2 problems here.

1- dmt is almost always present as a salt in plant material, so water hash or a butane extraction would not work.

2- do you know that the glands actually contain dmt? perhaps its the plant material that contains the dmt.

Just do an a/b on the plant material.
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Myco
#3 Posted : 7/15/2012 7:33:25 PM

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How do you know the glands produce DMT? What is the concentration of DMT in the gland heads?

If the content of the gland heads make up a large part of the present alkaloids then isolating them could indeed be worth the time.
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Xare
#4 Posted : 7/15/2012 7:34:46 PM

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The Day Tripper wrote:
I see 2 problems here.

1- dmt is almost always present as a salt in plant material, so water hash or a butane extraction would not work.

2- do you know that the glands actually contain dmt? perhaps its the plant material that contains the dmt.

Just do an a/b on the plant material.


Generally the essence of the plant is contained in the Trichomes.

I gave you the examples of cannabis, which contain THC. And Catnip that have nepetalactone. Another off the top of my head would be the trichomes of the tomato plant found on its stem and leaf that contain Tomatine, which is a Toxin and the reason that tomatos were once seen as poisonous. This is common all through nature.

These glands are specialized secretory structures of the plant that contain their essential oils and and resins. They are the primary sites of terpene accumulation.

My instinct tells me those crystal drops have the DMT.
 
Xare
#5 Posted : 7/15/2012 7:40:48 PM

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Myco wrote:
How do you know the glands produce DMT? What is the concentration of DMT in the gland heads?

If the content of the gland heads make up a large part of the present alkaloids then isolating them could indeed be worth the time.


The only way to know the concentration level for sure would be to test the Extract I plan to make from my materia.

But the extract is very pure, the bags filter out all the plant matter till you just have a gummy substance.

You can set it out to dry.

Or maybe you can further purify it by destruction the glands in a solvent and then doing a recrystallization.

Its a way to remove all the plant matter and have a very pure concentration of the essence of the plant, which iam certain contains the DMT because this is how all nature works.
 
endlessness
#6 Posted : 7/15/2012 11:37:43 PM

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Try out a couple different ways to see if you can isolate those glands. Then, extract from them. You can try to extract from the grass after the glands are removed, and compare the results. You can use some reagent such as marquis on the results of your extraction to try to get a clue at least if it has gramine mixed in or not.

You are supposing the glands have DMT but, appart from not yet know if its true that all/most alkaloids are in the phalaris glands, you dont know if that particular phalaris has any dmt to begin with, which is a major issue. Phalaris genetics is very variable, could be you have a strain with mostly gramine or similar potentially toxic alkaloids, or it could be its mostly 5-MeO-NMT or different beta carbolines of unknown activity, or maybe you're lucky and it's very high yield pure DMT or 5-MeO-DMT phalaris. So start extracting, take notes and pictures along the way, and let us know the results. Do NOT ingest something without being sure what it is. Keep us updated, and welcome to the Nexus!

(oh and, check the phalaris link in my signature if you havent yet)
 
Xare
#7 Posted : 7/16/2012 8:28:55 AM

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Yes iam aware of the Phenotype variation within a wild landrace of Phalaris grass.

A selective breeding process will have to be undertaken that isolates individual plants to grow them out and sample the essence of the plants until a DMT heavy phentotype is detected.

The same types of breeding programs are used within the cannabis scene to preserve certain characteristics of the plants like a High THC or CBD percentage within the glands.


There really is no other place for the DMT to be except contained within these plant secretory glands. Its not like the DMT comes out of the cellulose of the leaf.

A look into the plants Microcosm instantly revealed these living drops.
 
endlessness
#8 Posted : 7/16/2012 9:23:08 AM

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If you use as an example the weed, THC is not only present in the trichomes, though most is. Some is also in the plant part after the glands are removed.

How are you going to do a selective breeding (cloning) of the individual plants if you dont know which one has the desired alkaloids? The best would be if you would look into TLC, I think.
 
Parshvik Chintan
#9 Posted : 7/16/2012 9:56:37 AM

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i noticed some of the grass in more northern (relative to my location) parts of alaska have extremely prominent trichomes - to the point were they are noticeable from the naked eye at about 2 feet away.

the grass near me doesn't have any noticeable trichomes.

perhaps cold/22hr light can be the cause?
honestly i have no real idea.
just thought you might find it interesting.
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Infundibulum
#10 Posted : 7/16/2012 10:09:09 AM

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Xare wrote:
Yes iam aware of the Phenotype variation within a wild landrace of Phalaris grass.

A selective breeding process will have to be undertaken that isolates individual plants to grow them out and sample the essence of the plants until a DMT heavy phentotype is detected.

The same types of breeding programs are used within the cannabis scene to preserve certain characteristics of the plants like a High THC or CBD percentage within the glands.

These breeding programmes takes years and years fo dedication and many of the projects failing. Breeding programmes is not "easy" stuff.


Xare wrote:
There really is no other place for the DMT to be except contained within these plant secretory glands. Its not like the DMT comes out of the cellulose of the leaf.

I got problem with this absolute certainty of yours, so please drop it. You reach to a conclusion just by extrapolating your experience/knowledge from few other species...this kind of thinking very rarely correct. For dmt specifically (we do not care what other plants do with their secondary metabolites, remember, this is dmt we're discussing), you'll have to feed us with at least some indications that other dmt-containing plants od similar things. Acacias? chacruna? chaliponga? mimosa hostilis? desmanthus? do they also store their dmt in glands? If yes, then you're onto something. If not, you're really wasting your time.




Need to calculate between salts and freebases? Click here!
Need to calculate freebase or salt percentage at a given pH? Click here!

 
Xare
#11 Posted : 7/16/2012 10:12:28 AM

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endlessness wrote:
If you use as an example the weed, THC is not only present in the trichomes, though most is. Some is also in the plant part after the glands are removed.

How are you going to do a selective breeding (cloning) of the individual plants if you dont know which one has the desired alkaloids? The best would be if you would look into TLC, I think.




Well there is only trace amounts of the THC left in the plant matter after extraction.

Because its pretty much impossible to remove every single gland from the plant matter.

Or if you were using a solvent some of the solvent would wet your caput mortum and it would contain a bit of THC dissolved into the solvent.

So that would also account for the trace left over after extraction.


Most very good strains of cannabis have anywhere from 15-20 % of thc which is contained within the gland heads. So when you do an ice water extraction from nice starting plant material that is how much yield you end up with.

Iam working on the assumption that Reed Canary Grass will only yield about 0.120% of the dry plant matter. Maybe more since iam only going to use the leafs from the plant and not the stem which is mostly cellulose / fiber.



Since the plants are perennials they can self propagate through their root rhizome it will be fairly simple to Isolate Elite Individuals.

To do selective breeding I would have to visually inspect the plants for the traits that I deem worthy of keeping. I would use my microscope to look for certain plants that look like they might have the qualities we are after.

Like a very high yield % of trichomes to plant matter for starters.

But at some point the product would have to be tested to acctually see how much it contains and in what proportions.

A sample from each individual breeding selection would have to have tests carried out to see which one should be used a Parent Stock for further generations.


Usually when you are doing this for cannabis you go out into the wild and look for a landrace that has been growing by itself for many many generations. Then you take an assessment of that strain and look at the different phenotypes through the progeny.

If you see an outstanding individual that expresses the traits you want you ether take a cutting or save the seed from that plant.

But I dont know if iam brave enough to be the first ever to sample the pure resin of the Phalaris plant.

Id would rather have it tested so that we all know what it is Smile
 
Xare
#12 Posted : 7/16/2012 10:26:13 AM

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Parshvik Chintan wrote:
i noticed some of the grass in more northern (relative to my location) parts of alaska have extremely prominent trichomes - to the point were they are noticeable from the naked eye at about 2 feet away.

the grass near me doesn't have any noticeable trichomes.

perhaps cold/22hr light can be the cause?
honestly i have no real idea.
just thought you might find it interesting.


Hey that is pretty interesting !

I bet what you have there up north is a variety of the strain that expresses a characteristic of very large tricomes.

That would mean that you could get a better return % from that plant if you used it as starter material.
 
Xare
#13 Posted : 7/16/2012 10:45:24 AM

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200 grams of leaf have been collected. Its been kept in the freezer overnight.

And iam starting a small test ice water extraction right now.

 
endlessness
#14 Posted : 7/16/2012 11:02:44 AM

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Cannabis has THC on other parts of the plants not just the glands (Mahlberg & Kim 2008; Turner et al 1977; Fetterman et al 1971). Nevertheless they all note that the glands is where most cannabinoids are accumulated, as mentioned earlier.

I say this not to nitpick but just to prevent a yet unproven supposition ("all dmt is in phalaris glands" ) to prevent you from experimenting further and finding out if thats true or not. It might be phalaris works differently, it might be it works similar to cannabis, we dont know yet. As we all know, practice > theory, so let's try it out, attempt to isolate glands and extract, then extract from left over material, then we'll know Pleased

Regarding not wanting to be the guinea pig, I really suggest you get at least a marquis reagent or better, look into TLC, that way you can know what alkaloids are in your plant and even a semi-quantitative way to check for alkaloid content in different plants.

Let us know how it turns out Smile
 
Xare
#15 Posted : 7/16/2012 11:15:54 AM

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Right now iam giving the leaf a good 20 min mix in ice water to break free all the glands from the leaf surface.




Then I will pass this mixture through my extraction and collection bag kit.
 
Xare
#16 Posted : 7/16/2012 11:33:20 AM

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Now that the mixture is spun up and all the glands are in solution. Ive poured everything into my extraction bag kit and iam allowing it some time to settle.

Gravity will help to pull down the trichome glands that are floating in the water and they will pass through the screens of the larger bags until they get down to the smallest size bag. Where the screen should be smaller then the individual tricomes, allowing me to capture them.

 
Xare
#17 Posted : 7/16/2012 12:16:44 PM

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This is the result from the process.

It appears to me that the resin acted as a solvent upon the chlorophyll that was also released from the plant. So there is quite a bit of green contamination in the resin from the chlorophyll.

Ive seen this happen before but never so strongly.










These pics were taken just as it was taken out of the extraction bags and its still wet.

I have it on a screen to dry and ill take more pics of what it looks like dried.
 
Xare
#18 Posted : 7/16/2012 1:06:06 PM

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Ive used these same techniques for selective breeding with male cannabis plants.

As you know you cannot get buds from a male plant. But they do still produce some THC on the leafs. Ive looked at the glands on the surface of the male leaf with my small microscope before.


To do breeding with male plants normally you would have to select a few males that you like out of many seedlings. You would choose them by the traits that you like. For instance. How fast the plant grows, or the leaf blade look, or maybe its resistance to certain pests or fungus. These breeding considerations and more all go into what individual you will select for your parent stock.

But the final choice would be made after you smoked the leaf of the plant to find out what one is best.

Well instead of smoking a bunch of male plant leaf to see what male plant had a better more potent high. I would harvest all the leaf of the plant and make ice water hash from them.

Then I would try the extract from each of the individual males to base my final decision on what one I would use to Pollinate my females.


Sometimes when you stir the mixture for too long alot of the chlorophyll is released into the water and it makes you hash change color and to acquire a green tint.

But to guess, it looks like I got about a gram of extract with that run of the Reed Grass.

If I do another batch I will only spin it for about ten mins to keep the contamination down. The water looked fairly clear at the start then it got cloudy and slowly got greener and greener. So I think it was mixed for too long.
 
Xare
#19 Posted : 7/16/2012 1:31:52 PM

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Here are some 100x macro shots of the surface of the Phalaris Arundinacea Resin.

You can see how the living drops almost look like little stars all clustered together.












 
endlessness
#20 Posted : 7/16/2012 2:00:34 PM

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Why dont you basify that and try to extract?
 
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