Hello,

I am currently working on a theoretical tek for a crude extract of muscimol from Amanita muscaria. I've read the rules, and want to note that this is not an illicit drug, so I think this subject should be allowed here. It's an interesting subject from an organic chemistry perspective.

Well-dried Amanita muscaria were allowed to simmer in plain water. Both ibotenic acid and muscimol, the interesting compounds in this procedure, is water soluble. The heat should decarboxylate all the ibotenic acid into muscimol. The temperature was kept at max 60°C to prevent breakdown of muscimol, as it has a boiling point of 70°C. After an hour the mushrooms were filtered of, and the water was kept at a constant temperature of 60°C with a fan next to it for over two days, in order to reduce the solution to a resin.

Now, this resin is far from pure, and therefore I'm trying to find an easy way to clean it further. Muscimol has a pKa of 4.8, which means that at pH 2-3, 99.8-98.4% of the muscimol should be in salt form. By using 35% acetic acid, which have a pH of 2, the solution should be at pH ~2-3. Then one could pull with a non-polar, maybe heptane or perhaps d-limonene, which should pull the muscimol acetate. This could be basified and further cleaned if needed. Does this procedure seem correct? Will muscimol in fact become an acetate, and will it be pulled by a non-polar?

Perhaps I'm going at this the wrong way, maybe I should find a solvent that muscimol isn't soluble in, that will remove much of the other gunk? Maybe you have some other thoughts or theories? Please do share everything that comes to mind! Thanks in advance!

Additional bits of information: Muscimol is an extremely polar compound with a XLogP3 of -1.4. Muscimol is very soluble in water and methanol, slightly soluble in ethanol, DMSO and DMF.

Hello.

I am not a chemist but I do have lots of experience working with Amanita Muscaria and using it to magnify the effects of DMT and Ayahuasca..

I'll tell you what I have observed so far...

In my opinion the Amanita's MUST be sun dried in order for them to work properly...
And as far as I am aware....there are currently no vendors that sell sun dried Amanitas'.

I pick my Amanita myself at a high elevation in a mountain area. I then slice them very thin and let them dry out in super hot july style mid-day sunlight... If they don't finish in one day then I put them in the fridge until the next Sunny day and I do this until they are hard as crackers...

And so these things are so strong that I only have to take about 1/10th of a gram for it to work like a charm...once I took a half gram and it made my Ayahuasca trip way, way too strong it was like 20X normal strength...I wanted to freak out but the visuals were so vivid they managed to calm me just enough.

.I have tried the internet Amanitas and I thought they were horribly toxic giving some brutal stomach cramps and headaches also really not even actively magnifying the DMT...
I tried boiling them too but it did not work and it stunk to high heaven

So I think some chemical change caused by exposure to UV rays has to take place for the Amanita's to work right.

So I'm thinking the Mushrooms need to be sun dried BEFORE you can extract anything worthwhile.

I have also done like a everclear rinse of sundried powdered amanita..this seemed to make the amanita even more smooth with less stomach issues.

Muscimol extraction is something very hard to achieve. Nothing as easy as n,n-DMT or Mescaline.
When trying to find a way to extract it, what you need as a minimum is Thin Layer Chromatography - to analyse your pulls etc... so you know where the muscimol is and where it goes from there.

But, there has to be a way of extracting it without Chromatography. Im sure its not complicated either. We just have to figure it out.

So, from what I remember...

Johnathan Ott supplemented his income some ~30 years ago by doing ibotenic acid extractions from Amanitas. He sold his extract for something like $5000 per gram to pharmaceutical companies. Iirc, the high price was a result of the amount of material needed and difficulty/lengthiness of the extraction.

Ultimately, I don't think you're going to be able to work it past a crude extraction, but I'd suggest looking for scientific articles on the subject if you really want to hack away at it.

Muscimol goes for about 50 G's a gram. But I have had a crude acetate resin from amanitas before.

Ion exchange column chromatography is what Ott used I believe. I'm sure there are other ways but I can't think of anyway around chromatography.

Who's buying muscimol? Let's start a small-scale target synthesis company, please.

I don't really see the point of going through all that trouble unless it's for science or to make $$ (I can't believe it's that valuable..

Correct me if I am wrong but I really doubt that "pure" muscimol would be in any way safer or better than just eating a piece of the mushroom.

It would be highly beneficial for several reasons. One being the fact that the potency varies greatly from mushroom to mushroom and two, the fact that they make you sick.

goddywoddy wrote:



I'm not trying to burst your bubble here but I'm afraid even pure Muscimol makes you sick..

Have a look:
Muscimol Data Sheet PDF.


If you living in a place where Amanita grows then there is no reason you cannot harvest your own extremely potent Amanitas....

They are most potent during the peak of the summer season as far as I am aware....Also even if the ones you find are weak, if you find them growing you could probably get and entire grocery bag full of the things so I think you will have enough, so it's not like they are a rarity or anything...at least where I live.

The ones that I pick are so unbelievably strong and they do not make me sick AT ALL unless I take WAY too much...I have taken sun dried Amanita more than 40 times and I have not gotten sick any of those times.

of course I only use them to magnify other psychedelics...as far as I have experienced they only make you sleepy and sort of stoned feeling even at high doses by themselves.....They will make your dreams incredible though.

If you are trying to trip off of just Amanitas by themselves I think you will be pretty disappointed by the results...Now if you combine psilocybe mushrooms with Amanita THEN you will have something to write home about.

i agree entirely, just because it hasnt been done, dosent it mean it cant be.

theres probably some relatively simple procedure that yields a product we desire, just keep pluggin away!

Anyone tried just soaking amanitas in ipa?

I believe ethanol would work better than ipa

currently i have 1 cap of peeled off skin soaking in ipa, it seems to be a yellow amanita, mew thinks it wont kill him but he is less than sure...