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Griffonia Simplicifolia extraction Options
 
arimane
#1 Posted : 3/29/2012 7:00:05 PM

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Dear nexus,

I'm now approaching the graduation in Herbalism, a university degree here in Italy, so I'm starting to write my thesis.

It will be about serotonin precursor 5-htp, the Griffonia Simplicifolia etract, as antidepressant and for weight loss.

I would like to make a nice job, as many can understand here I'm in love for serotonin, and would like to homage my favorite and most enlightening neurotrasmittor with this thesis.

So I thought that maybe you guys have some nice ethnobotanical datas, link, ebooks, I'd like to make a good chapter about it, or if you know something interesting about this plant, please post it.

Thanks,

Ari
Bad, bad english
 

Live plants. Sustainable, ethically sourced, native American owned.
 
arimane
#2 Posted : 4/17/2012 11:05:13 PM

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Sorry for bump, I would like to know how would you approach an extraction of 5-htp from Griffonia seeds... since is not an alcaloid, and I've never extracted an aminoacid, I'd really appreciate some help.
Thanks!
Bad, bad english
 
Dante
#3 Posted : 4/17/2012 11:47:35 PM

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chiedi a benzyme!

buona fortuna
Listen to a man of experience: thou wilt learn more in the woods than in books. Trees and stones will teach thee more than thou canst acquire from the mouth of a master. St. Bernard
 
Infundibulum
#4 Posted : 4/18/2012 3:25:46 AM

Kalt und Heiß, Schwarz und Rot, Kürper und Geist, Liebe und Chaos

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arimane wrote:
Sorry for bump, I would like to know how would you approach an extraction of 5-htp from Griffonia seeds... since is not an alcaloid, and I've never extracted an aminoacid, I'd really appreciate some help.
Thanks!

Aminoacids are zwitterionic molecules; as their name indicates, they are both bases and acids. Extracting an amino acid is easy, just make an aqueous extract, or tincture etc...Isolating an aminoacid to relative purity requires mastery of separation techniques - you'll most definitely need a chromatographic separation in the beginning followed by a isoelectric focusing of your amino acid of interest in a pH gradient - all of which tough stuff to do at home!

Benzyme could definitely offer more insight on the technical side but still the consensus will be that it'll be tough business to undertake such a task.


Need to calculate between salts and freebases? Click here!
Need to calculate freebase or salt percentage at a given pH? Click here!

 
arimane
#5 Posted : 4/18/2012 4:46:00 PM

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You're right, Infundibulum, I ment isolating, not extracting.
Unfortunatly, I can do a TLC, but I don't have the tools to do an HPLC... hope it's not strictly needed. I could start with a defat, an idroalcolic extraction but then...

I don't want to disturb Benzyme with a pm, hope he'll get to see the topic.

If I get to some results I'll post.
Bad, bad english
 
 
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