Is it possible to use the natural plant enzymes to help DMT yield?

The answer could of course be a simple "no". Is this answer known? If not, it could be fun to investigate this.

For example, we could try to release the plant enzymes naturally present, and then add inputs (over the counter amino acids and enzyme cofactors) to try to get the reaction going at biological conditions (gentle pH/temp). As DWFZ points out, one way of this not working is if the enzymes needed are not present by default and waiting for gene expression.

In sone Teks like CYB's max ion, it has been found that freeze/warm water cycles of the ground submerged bark could be beneficial for yields. Cell lysing will help release DMT directly, but what is the purpose of the warm cycle? If enzymes are released during the freeze, could some extra DMT biosynthesis occurs during the warm step?

It that is the case, then adding tryptophan, P5P and SAMe could help the yield boost from the freeze/warm cycles (all over the counter health supplement)

Does anyone know why this won't work? Is it worth a try?

Lets see what happens 🙃

150g bark covered in water, Freeze/thaw (see first image), added tryptophan (3.6g) + P5P (250mg) + SAMe (15.6g) and set at a warm temp (40C) for a few hours with stirring. SAMe turned the bark an ochre red (see second image image).

Will extract this brew as usual and see what kind of yield results 🤞

Thanks for the desire to do innovative research and share with us!

Very interesting indeed!

Nice, glad to see you're taking practical steps - and with a simpler reaction than the mescaline boosting idea.

Thanks. One additional difficulty with mescaline is that one of the enzyme cofactors (Tetrahydrobiopterin) is only available by prescription. This is not an issue for DMT, where the inputs and cofactors are all available over the counter for me (but we still need active enzymes to be supplied by the bark of course and that is an unknown).

The extraction is coming along, but has been a little messy; the supplements influenced the extraction. Thankfully it is all clearing up with an extra A/B step.

Ok, got 3095mg of fluffy white spice (2.1% yield). Extra cleanup steps needed. I think the SAMe supplement added gunk to the extraction.

Based on the yield there is no evidence of enzymatic conversion of the supplements to DMT. However, before adding SAMe, there was light bubbling so perhaps some decarboxylation of tryptophan with CO2 release happened. The addition of SAMe supplement with all it's extra ingredients dropped the pH very low and may have been an issue.

In chat it was mentioned that when brewing beer, the grains that are freshly milled have more active enzymes than older grain. Therefore, another issue is that the bark could have been too old.

Or of course, this may never work, even if using fresh plant material and keeping the pH in the biological range and.

Next steps: I want to repeat this at least once again with two changes: (1) Fresh leaves from chacruna plants will be used, and (2) SAMe supplement will be dissolved in water and the pH adjusted to biological conditions before adding it to the plant extract.