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Creachemist
#1 Posted : 11/8/2019 4:11:51 PM
Hi swims and swimettes

I am planning to do a methanol extraction on cubensis and want te perform a chromatography but for me it is pretty impossible to get chloroform and benzene, so my question is, is the pre wash with those chemicals essential to perform a chromatography or is it only harder without it?

Thanks for the ones that can help me!
 
burnt
Extreme Chemical expertChemical expertSenior Member
#2 Posted : 11/8/2019 4:42:22 PM
What is the solid phase? Fine silica gel? Are you referencing a particular study? If they used benzene and chloroform its probably old and outdated. There are other ways.

 
Creachemist
#3 Posted : 11/8/2019 4:52:12 PM
burnt wrote:
What is the solid phase? Fine silica gel? Are you referencing a particular study? If they used benzene and chloroform its probably old and outdated. There are other ways.



Yes or cotton if that is possible but probably silica, i am refearing to most teks i have read i was already planning on a heptane de-fatting but since i am not a chemist i would love if you can give me some feedback (note i am not a chemist so i cant achieve some of the more heavy chemicals)

Thank you!
 
 
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