A while back an analysis was done on two samples, one without re-x and the other with. Toluene was used as solvent, re-x in hexane. The first result displays two major peaks. If the main one is N, N - dmt, what would the lesser be? The UV absorption spectra for both is different, but not sure how to read it in order to say what those differences mean.. The second result shows one main peak. If this too is N, N - dmt, why is the absorption spectrum different than the major peak of the first test? Also included the data from the raw sample before dilutions were known to be necessary. Toluene pulls up so much more than ever would have expected! Samvidbuho attached the following image(s):  Test 1, 280nm (report style).JPG (19kb) downloaded 47 time(s). Test 1, %area of peaks.JPG (32kb) downloaded 47 time(s). Test 1, 227nm signal analysis II.JPG (79kb) downloaded 47 time(s). Test 1, 227nm signal analysis I.JPG (79kb) downloaded 47 time(s). Test 2, Run 6 280nm (report style).JPG (19kb) downloaded 47 time(s). Test 2, Run 6, 227 %area of peaks.JPG (31kb) downloaded 47 time(s). Test 2, Run 6, 227nm spectral I.JPG (53kb) downloaded 47 time(s). Test run 2, undiluted.JPG (141kb) downloaded 47 time(s).
|
|
|
Im not at home now to check but i'd bet it's NMT (monomethyl tryptamine). Try to see if you can find the spectra in literature and compare.
|
|
|
Another possibility might be 2-methyltetrahydro-β-carboline. What was the source of your analytical sample? “There is a way of manipulating matter and energy so as to produce what modern scientists call 'a field of force'. The field acts on the observer and puts him in a privileged position vis-à-vis the universe. From this position he has access to the realities which are ordinarily hidden from us by time and space, matter and energy. This is what we call the Great Work." ― Jacques Bergier, quoting Fulcanelli
|
|
|
Oh interesting. I can't find anything in the references I have that give the UV spectrum for NMT. I'll keep looking.
Source was mimosa hostilis, dried and powderized.
|
|
|
what are the mobile phases, and what is the stationary phase (the column)? Retention times can differ from either pH or polarity. "Nothing is true, everything is permitted." ~ hassan i sabbah
"Experiments are the only means of attaining knowledge at our disposal. The rest is poetry, imagination." -Max Planck
|
|
|
Mobile phase A was H2O + 0.1% TFA,
Mobile phase B was methanol,
Ran gradient of 5% B to 50% B over 17.5 min.
Column was a Zorbax C18.
|
|
|
Thinking about it now, using an acidic mobile phase doesn't make sense, right?
|
|
|
From Clarke's Analysis of Drugs and Poisons, The 3rd / last spectrum I posted looks like the reference one for N,N DMT.. Samvidbuho attached the following image(s): Screenshot_20190316-195317_EBookDroid.jpg (50kb) downloaded 19 time(s). Screenshot_20190316-195356_Gallery.jpg (59kb) downloaded 19 time(s).
|