Why not 100°C ? And were you baking fresh mushrooms ? If so did you tried to just lightly bake them and collect only the juice, juice that can be drunk as is or dried for later use (eventually gelcaped).

WIRKSTOFFE AUS AMANITA MUSCARIA :
IBOTENSAEURE UND MUSCAZON

Die allgemein bekannten Wirkstoffe des Fliegenpilzes,
wie Muscarin, Acetycholin und Muscaridin bieten keine
Erklärung für das Gesamtbild der Fliegenpilzvergiftung
(1). Wir haben deshalb schon vor längerer Zeit nach
weiteren, physiologisch aktiven inhaltsstoffen zu suchen
begonnen. Eine breite pharmakologische Prüfung der
wasserlöslichen Anteile ergab das Vorliegen von
Fraktionen mit starker narkosepotenzierender Wirkung.
Zur präparativen Isolierung diente ein entfetteter
Alkoholextrakt aus Amanita muscaria, der besonders
schonend aufgearbeitet worden war. Durch sorgfältige
Trennung an Dowex 50 W x 12 (H) mit wasser und
verdünnter Ameisensäure erhielten wir kristallisierte
Ibotensäure (2) Smp. 145 (Zers.) , C5H8N2O5 und
Muscazon, Smp. > 190 (Zers.) , C5H6N2O4 . Beide
Verbindungen reagieren mit Ninhydrin auf Papier gelb
und geben mit Grote’s reagens blaue Flecken.

Die Ausbeute an Ibotensäure betrug in der Ernte 196?
215g aus ca. 700 kg Frischpilzen, d.h. 0,03 %. Der
wirkliche Gehalt darf auf 0,05 % geschätzt werden,
sodass Ibotensäure wegen ihrer hohen
pharmakodynamischen wirksamkeit als wesentlicher
inhaltsstoff von Amanita muscaria angesehen werden
darf.

Nach weniger schonender Aufarbeitung isolierten wir
196? einen Wirkstoff , C4H6N2O2, Smp. 174-175 , aus
wasser - methanol, welcher sich als Abbauprodukt von
Ibotensäure erwies.




ACTIVE SUBSTANCES FROM AMANITA
MUSCARIA: IBOTENSAEURE AND MUSCAZON
Those generally did not admit active substances the fly
mushroom, like Muscarin, Acetycholin and Muscaridin
offer an explanation for the overall view of the fly
mushroom poisoning (1). We therefore already began to
look before longer time for further, physiologically active
contents materials. A broad pharmakologische
examination of the water-soluble portions resulted in
being present parliamentary groups with strong
narkosepotenzierender effect. For the praeparativen
isolation a degreased alcohol excerpt from Amanita
served muscaria, which had been particularly carefully
regenerated. By careful separation at Dowex 50 W x 12
(H) with water and diluted formic acid we received
crystallized Ibotensaeure (2) Smp. 145 (Zers.) ,
C5H8N2O5 and Muscazon, Smp. 190 (Zers.) ,
C5H6N2O4. Both connections react with Ninhydrin to
paper yellow and give with Grote's reagent blue marks.
The yield at Ibotensaeure amounted to in the harvest
196? 215g out approx.. 700 kg fresh mushrooms, i.e.
0.03 %. the real content may become estimated on 0,05
%, so that Ibotensaeure may be regarded because of their
high pharmacodynamic effectiveness as substantial
contents material of Amanita muscaria. After less careful
processing we isolated 196? an active substance,
C4H6N2O2, Smp. 174-175, from water - methanol,
which proved as dismantling product of Ibotensaeure.



paper 2:


A novel constituent of amanita muscaria


isolation of 3-hydroxyisoxazole from natural sources.

fresh plants were added to boiling water, cooled rapidly,
filtered and the filtrates treated with Deacidite FF (base)
resin. The resin was washed with water and the
compound eluted by means of acetic acid.The eluate was
freeze-dried, disolved in water and passed down a
column of celulose phosphate (whatman P70). the
substance gave a pale yellow band which was elated with
N NH4OH and the eluate freeze dried . The
semi-purified material was crystallized from alcohol to
give the compound, mp. 172-174 (decomp)

Next bioessay:

Transdermal muscimol

Preparing the Holy Oil
Olive oil and animal fat are both absorbed into the skin. As far as preservability
goes, olive oil has an edge over animal fat, especially in warm or hot conditions.
The active principal of the herb can also be absorbed into the skin; if you gently
hold a fresh Amanita Muscaria stem in your hand for about 5 minutes, you will
start feeling a light giddiness, the longer the contact, the more intense the effects. [this claim seems a more than a bit dubious]

To create the holy oil, powder some dried specimens and sift the powder
through a fine cloth. Return the pieces that didn’t pass to being repowdered. Once
you have the desired amount of fine herb powder, place into a clean dry small
sealable vial or bottle. Slowly add just enough olive oil to cover the powder.
Using a piece of wire, gently stir the powder and olive oil together. Add more oil
as needed to create a thin paste. Seal the container and let the herb soak in the oil
for an hour or two. To use, place a fingertip on the surface of the oil and apply to
the center of the forehead about an inch or so above the eyebrows. The effects
should start in a few minutes and last until the oil is all absorbed. This is the most
controllable method of using dried Amanita Muscaria and probably the most
efficient way of using a limited quantity of dried mushroom for a number of
people. The effects are never too intense unless too much oil was applied

This oil is also a very effective topical antibiotic and can be used on many
different infections of the skin; such as inflamed insect bites or other infected
wounds.