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Protein binding [does it effect the experience] Options
 
rawmo
#1 Posted : 6/1/2010 12:01:11 AM
Hey there,

just seeing if anyone has done any experiments / comparisons re protein binding [egg whites] of tanins in Mimosa ayahuasca?

It seems to me from my experimentation that there is considerable less nausea, but that you need more MHRB in the mix to get the same intensity.
[or that it gives a qualitatively different experience]

I'm aiming to try a non egg white aya in the next few weeks, but was just seeing if anyone else had done any tests [with/without tanin binding] and could offer their knowledge/experience re it.

cheers : )
 
ms_manic_minxx
Moderator
#2 Posted : 6/7/2010 7:20:52 AM
I don't dabble with eggs (I cracked a bloody one once and it ruined them forever for me...), but, do you allow your Mimosa to decant before drinking? Decant for at least one day, or until a thick sludge forms on the bottom: there should be almost no Mimosa-related nausea this way. Smile
Some things will come easy, some will be a test
 
69ron
#3 Posted : 6/7/2010 7:55:27 AM
Dagger wrote:
I prefer to use gelatin on mimosa. The difference is huge imho. After fining with gelatin, there is no astringency left. After that the mimosa isn't bad at all to drink. And no noticable loss of actives.


How much gelatin? What's the procedure?
You may remember me as 69Ron. I was suspended years ago for selling bunk products under false pretenses. I try to sneak back from time to time under different names, but unfortunately, the moderators of the DMT-Nexus are infinitely smarter than I am.

If you see me at the waterpark, please say hello. I'll be the delusional 50 something in the American flag Speedo, oiling up his monster guns while responding to imaginary requests for selfies from invisible teenage girls.
 
rawmo
#4 Posted : 6/7/2010 11:05:28 AM
69ron wrote:
How much gelatin? What's the procedure?


yeah i second that,

any info much appreciated

[my initial tests with gelatin just resulted in jelly, and i'd rather not ingest gelatin either if possible]
 
69ron
#5 Posted : 6/7/2010 7:30:07 PM
Dagger wrote:
Only thing is that I am not sure if it removes the actives too...


Thanks for posting the details!

For sure some is removed. Nearly all cleanup steps remove some alkaloids. The question is how much?
You may remember me as 69Ron. I was suspended years ago for selling bunk products under false pretenses. I try to sneak back from time to time under different names, but unfortunately, the moderators of the DMT-Nexus are infinitely smarter than I am.

If you see me at the waterpark, please say hello. I'll be the delusional 50 something in the American flag Speedo, oiling up his monster guns while responding to imaginary requests for selfies from invisible teenage girls.
 
rawmo
#6 Posted : 6/26/2010 6:31:20 AM
Quote:
After it has settled, you can remove the clear liquid into another container. You can also squeeze the precipitates for liquid, it is quite easy to filter. The precipitates can be discarded.


how long does it take to settle?

I'm just in the process of trying a gelatin tannin binding.

32g Mimosa
5g Gelatin [in solution]. Decided to start lower on the % that 30% in Daggers recommendation just to see.

The mixture went cloudy straight away as soon as I started adding and stirring,
Stayed that way.
I've done an extra filtration step on it [through cotton wool] which seems to have grabbed some darker purple material in the filter [which may be a combo of bark and precipitate?]

But the solution has stayed cloudy pink
No real stress I suppose, but i think clear may have looked prettier clear Smile

Anyway,
To reconfirm -
1] How long should you expect the solution to take to settle precipitates to the bottom of the container?
2] Will this be faster [any ideas?] at warmer or cold temps. I'd imagine cold / in the fridge (less convection) but always worth checking

thanks
 
rawmo
#7 Posted : 6/28/2010 4:41:46 AM
Quote:

Ouch. Cloudy pink is a sign that you have used too much. Will be hard to clear it now. Ideally it should clrash out very quickly into big chunks. Settling should be very fast, although complete settling can take some hours.

How many percent did you use?

15%
It did crash out after 20 or so minutes : )

Quote:
And what was the temperature on the brew you added it to?

~50 degrees C
Quote:
How much liquid was in the brew?

~235-250ml (went to ~200ml after the tannin binding)
Quote:
Btw, did you add the gelatin solution to an already clear brew? Or did you have pieces of bark in it? I have always done it on a clear brew.

Yup, filtered out the powdered bark before (using a fine mesh filter) before adding Gelatin.

One thing I noticed [on a dose of 50g Caapi, 8g Mimosa for 75kg, 2x 85Kg body weight] was that it wasn't very strong effect wise. Especially compared to the previous aya with the same ratio (but using egg white for protein binding instead of Gelatin).
At a guess I'd probably use 12g Mimosa for that body weight in future.
6.5g for bodyweight 67kg person from the same extraction was a strong trip (at peak open/closed eyes making no difference)

So in conclusion it would seem that while an extremely effective tannin binder [not even a hint of nausea for anyone], the gelatin does cause loss of some of the actives (if the precipitate is discarded... even when it all moisture was pressed out).

Any extra wisdom much appreciated.
 
rawmo
#8 Posted : 6/28/2010 8:10:49 AM
Dagger wrote:
I can't say I have experienced any loss of actives with this method. I need less that 1 gram of mimosa for a good dose. I usually do it with a room temperature brew. I also put it in a THP setup and send through some water to pick up actives that may have been stuck in the precipitates.


wow 1g!, that seems really low from my understanding of amount of actives [that would be 5-10mg tops, not including what is lost in the extraction from my understanding].
With 1g do you get the full aya 'dream' experience [as in DMT like visuals, and less abstract vistas as well], 1-2 hrs of in deep experience, open eye patterning etc
i.e. how strong is the journey on 1g mimosa? on a 1-5 scale where 1 is a light dusting, 3 is a full trip (with moments of passing through the veil into the scenes you see and 5 is a heroic dose where you totally loose perception of this reality for large amounts of the journey?

I found 6.5g mimosa with no protein binding was quite strong (visual and audio), my strongest trip yet on aya (but still totally manageable), but the nausea (and upset stomach for 1.5 days after) made me hesitant to go through that again in a hurry.
Hence experimenting with tannin binding options.


Quote:
Btw, would you say that you prefer gelatin over egg whites when it comes to nausea and taste?

yeah qualitatively, and from 3 tests egg white, 3 gelatin, Gelatin is better for the following reasons
1] easier to work with [make a solution, add to filtered brew], remove solids
2] considerably better taste profile (almost pleasant)
3] way less nausea (i.e. none at all)
Being vegetarian i do have some moral issues with it, but the aya certainly doesn't seem to mind any ; )

As an extra question for your room temp extraction in THP.
how much water do you add per gram?
my thoughts are that with powdered mimosa most of the actives should come out quickly and with not too much water,
[e.g. the noman tek requires only ~700 ml / 50g]
If we could work out how much water maximum needs to be used for powder to get a good [complete] extraction it could save hours of boiling.

btw - cheers for the feedback, ideas and advice

 
rawmo
#9 Posted : 7/16/2010 4:13:35 AM
Is it worth perhaps putting up a wiki for tannin binding for aya?

I'm happy to collate the different ways, then post it to this link for any feedback/corrections.

lemme know if you think it is worthwhile?
 
endlessness
Moderator
#10 Posted : 7/16/2010 4:40:35 AM
I think its worth it Smile
 
 
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