Just got this to control the hplc pump/run the gradients, and it can also control/record signals from the absorbance detector I have from a different manufacturer (ABI 785A). the software manual is 3028 pages.

the current setup is nearly identical to what I used to analyze IH's harmalas and Jork's p.brachy seedlings...and my own c.paspali experiments, in 2010.

sensitivity is noticeably improved. I switched from orthogonal electrospray, with a sample tube of 100 um i.d., to capillary nanospray, 20 um i.d.

20 micron i.d. fused quartz silica capillary I flame-pulled to ~5 micron i.d., and cut with a ceramic blade. 100X mag., Zeiss KF 2

Holy crap! What would you use such a small tube for?

low-flowrate/high sensitivity LC-MS.

I am reproducing what I remember from experiments I conducted 7 years ago in college, from my man cave.
I never actually prepared emitter tips, like I am doing now. they were purchased.
getting down to this level of sensitivity, analytes from intracellular serum may be characterized....from a single cell.


ideally, the tip would have more of a taper, and that is done with a laser.

Intracellular serum.. those are some choice words. !

Images from a spectroscope while teaching a basic spectroscopy lab. Two of the four lines of visible light that make up the Balmer Series of the Hydrogen atom.

Very nice benzyme for something so delicate

Is this your first attempt at doing something like this?

second attempt with 20 um. been practicing with 75 um.
yea, this is a recent practice, since I swapped out the ion source.
I have much greater control over the plume entering the ion transfer capillary,
and spectra like this is typical now.

very easy to utilize capillary, because it's translucent, and can be troubleshot under a microscope.
I just switch out lines as needed.

danng, real nice..

out n about the other morning

.

My other love apart from psychedelics and entheogens.

Cracking german DMEs and hours of tuning and adjusting various tables/values for maximum power and efficiency.

that looks quite technical.
can you explain abit what this is about? I assume its about engines? definitly something I have abolutely no clue about.

But when someone states something is their passion, I always get interested

I agree. That looks interesting Tatt. I too would like to hear more. Is it something to do with dimethyl ether and direct injection engines?

I could spend days talking this, though in the same respect i'm a newb with much of this stuff compared to many out there, especially the big names. I'll gloss over these photos to give a small rundown:

The program in those photos is TunerPro, one of the best pieces of tuning software out there, especially when it comes to german and bavarian automotive electronics.

The top photo is the various tables I have open for torque requests that the transmission/transmission control unit sees from the DME[ECU]. Torque request values can be altered per the specific AT [automatic transmission], which can have a slew of various changes - upshift speed from gear to gear, torque specifics per gear/RPM/load, pedal sensitivity, etc.

Second photo down is timing which is ignition timing values. On the piston’s decent from the top of the cylinder, intake valves are opened allowing the cylinder to fill with air and fuel. After the cylinder is filled with air, it makes its ascent towards the top of the cylinder in what is referred to as the compression stroke. Towards the top of the compression stroke is where the spark plug fires, igniting the compressed air/fuel, which drives the piston back down (power stroke), and of course the piston’s next ascent pushes the burnt gases out of the exhaust valves (exhaust stroke). By adjusting the values in the tables you are essentially adjusting the degrees BTDC [before top dead center] that the spark plugs fire, allowing it to be not too soon or too late, but right at the specific degree to where the spark ignites the air/fuel mixture with perfect efficiency.

The next pic/table down is WGDC [wastegate duty cycle]. On forced induction [turbocharged vehicles] attached via the turbocharger/manifold is whats called a wastegate, which regulates the boost[air] pressure at which exhaust gases pass ny the turbine by opening or closing a bleeder vent. This prevents overboost and/or excess buildup of unsafe boost levels, and also regulates boost stability. WGDC is typically only a parameter on higher end turbocharged vehicles [in the case of those tables a BMW 335i] - it's basically a signal that the DME[ECU] sends to the wastegate diaphragm to regulate the required bleed off.

There's pages I could write that cover these few things I pointed out. Those are just a couple of the tuning parameters when getting into these german/bavarian autos, not to mention the near endless amount of DME algorithms, opened and closed loop tables and systems that higher end vehicles like BMW and Mercedes employ, also the ability of the DMEs in these specific cars to adapt on the fly and produce brand new algorithms basically at any given moment.

Cool Tatt, looks like an enormous iterating process, with a never-done effect.
After carburetors and Delco's left the stage and ECU's came in, this kind of tuning work is like a black art. It's amazing how they over time, could reduce exhaust gasses toxic amounts continually and still augmenting the obtained performance out of a given amount of fuel. There's a thing of respect for resources/environment connected to it.

Oops, digress off topic